Summary
Many attempts have been made to culture the stem cells that are known to reside at the base of the intestinal crypt. However, no successful method for the long-term culture of these cells has yet been published. We wish to describe a method that maintains human colonic crypt cells in a viable, slowly proliferative state for more than 3 mo. The method involves the use of a viable bovine aortic endothelial cell feeder layer that limits its potential for the study of potential growth factors for the colonic cells.
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Whitehead, R.H., van Eeden, P.E. A method for the prolonged culture of colonic epithelial cells. Journal of Tissue Culture Methods 13, 103–106 (1991). https://doi.org/10.1007/BF01666139
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DOI: https://doi.org/10.1007/BF01666139