Summary
Techniques are described for the culture of murine embryonic stem cells in the absence of heterologous feeder cells and for the induction of differentiation programs. The regulatory factor differentiation inhibiting activity/ leukaemia inhibitory factor (DIA/LIF) is produced at high concentration by transient expression in Cos cells and is used to suppress stem cell differentiation by addition to the culture medium. Differentiation is then induced in a controlled manner either by withdrawal of DIA/LIF or by exposure to the chemical inducers retinoic acid or 3-methoxybenzamide.
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Smith, A.G. Culture and differentiation of embryonic stem cells. Journal of Tissue Culture Methods 13, 89–94 (1991). https://doi.org/10.1007/BF01666137
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DOI: https://doi.org/10.1007/BF01666137