Summary
Strains ofEscherichia coli resistant to ceftazidime but susceptible to other third generation cephalosporins were detected in three patients at two different locations (München, Bremen). The resistance was self-transmissible to otherE. coli strains. Resistance against ampicillin, kanamycin, chloramphenicol and sulfonamide was co-transferred. The isoelectric point (pI) of the β-lactamase was similar to the pI of the AER-1 and LCR-1 β-lactamases. These enzymes, however, do not confer resistance to ceftazidime. Therefore the β-lactamase described is the first example for a ceftazidimase.
Zusammenfassung
Ceftazidimresistente Stämme vonEscherichia coli wurden von drei verschiedenen Patienten an zwei Orten (München, Bremen) isoliert. Die Stämme waren empfindlich gegenüber anderen Cephalosporinen der dritten Generation. Die Resistenzdeterminante ist auf einem R-(Resistenz-)faktor lokalisiert und wird mit Resistenz gegenüber Ampicillin, Kanamycin, Chloramphenicol und Sulfonamid cotransferiert. Der isoelektrische Punkt der β-Laktamase liegt bei 5,9. Aufgrund der spezifischen Aktivität gegenüber Ceftazidim (Ceftazidimase) ist diese β-Laktamase von allen bisher bekannten Cephalosporinasen verschieden.
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Literature
Shah, P. M., Stille, W. Escherichia coli andKlebsiella pneumoniae strains more susceptible to cefoxitin than to third generation cephalosporins. J. Antimicrob. Chemother. 11 (1983) 597–601.
Bauernfeind, A., Shah, P., Petermüller, C., Motz, M.: Plasmid-determined resistance to third generation cephalosporins in Enterobacteria. Chemioterapia, Suppl. to no. 2 (1985) 30–31.
Hedges, R. W., Medeiros, A. A., Cohenford, M., Jacoby, G. A. Genetic and biochemical properties of AER-1, a novel carbenicillin-hydrolyzing β-lactamase fromAeromonas hydrophila. Antimicrob. Agents Chemother. 27 (1985) 479–484.
Simpson, I. N., Plested, S. J., Budin-Jones, M. J., Lees, J., Hedges, R. W., Jacoby, G. A. Characterisation of a novel plasmid-mediated β-lactamase and its contribution to β-lactam resistance inPseudomonas aeruginosa. FEMS Microbiology Letters 19 (1983) 23–27.
Matthew, M., Harris, A. M., Marshall, M. J., Ross, G. W. The use of analytical isoelectric focusing for detection and identification of β-lactamases. J. Gen. Microbiol. 88 (1975) 169–178.
Sanders, C. C., Sanders Jr., W. E., Moland, E. S. Characterization of beta-lactamasesin situ on polyacrylamide gels. Antimicrob. Agents Chemother. 30 (1986) 951–952.
Bauernfeind, A. Classification of β-lactamases. Rev. Inf. Diseases 8, Suppl. 5 (1986) 470–481.
Medeiros, A. A. β-lactamases. Br. Med. Bull. 40 (1984) 18–27.
Kliebe, C., Nies, B. A., Meyer, J. F., Tolxdorff-Neutzling, R. M., Wiedemann, B. Evolution of plasmid-coded-resistance to broad-spectrum cephalosporins. Antimicrob. Agents Chemother. 28 (1985) 302–307.
Goldstein, F. W., Kitzis, M.-D., Acar, J. F., Gutmann, L.: Plasmid-mediated-resistance to 3rd generation cephalosporins in Enterobacteriaceae. Abstracts of the Third European Congress of Clinical Microbiology. Den Haag (1987) Abstract no. 210.
Sirot, D., Sirot, J., Labia, R., Morand, A., Courvalin, P., Carfeuille-Michaud, A., Perroux, R., Cluzel, R.: Transferable resistance to third-generation cephalosporins in clinical isolates ofKlebsiella pneumoniae: Identification of CTX-1, a novel β-lactamase. J. Antimicrob. Chemother. (1987) in press.
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Bauernfeind, A., Hörl, G. Novel R-factor borne β-lactamase of Escherichia coli confering resistance to cephalosporins. Infection 15, 257–259 (1987). https://doi.org/10.1007/BF01644127
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DOI: https://doi.org/10.1007/BF01644127