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Die In-vitro-Aktivität von Mezlocillin, Azlocillin und Carbenicillin gegen Bacteroidaceae unter besonderer Berücksichtigung der Fragilis-Gruppe

In vitro activity of mezlocillin, azlocillin and carbenicillin against bacteroidaceae with particular reference to bacteroides fragilis

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Zusammenfassung

In Röhrchenverdünnungsversuchen wurden ca. 90 Bacteroidaceae-Stämme gegen die neuen Breitbandpenicilline Mezlocillin und Azlocillin sowie vergleichend gegen Carbenicillin geprüft. Unter dem Teststammkollektiv waren vor allem die pathogenetisch besonders relevanten und alsβ-Laktamase-Bildner bekannten Arten Bacteroides fragilis und Bacteroides thetaiotaomicron vertreten. Durch Mezlocillin-Konzentrationen von 16µg/ml oder darunter wurden 81% und durch 32µg Mezlocillin/ml über 96% der Bacteroides-Kulturen inhibiert. Azlocillin-Konzentrationen von 16µg/ml oder darunter hemmten fast 67% und 32µg Azlocillin /ml etwa 93% der Bacteroides-Stämme. Ähnliche Hemmquoten von 55% bzw. 87% und 94% erzielte Carbenicillin erst bei 32 bzw. 64 und 256µg/ml. Gegenüber Keimen der Sphaerophorus-Gruppe erbrachten Mezlocillin und Azlocillin im Vergleich mit Carbenicillin keine wesentlichen Vorteile. Die statistische Analyse der Beziehung zwischen minimaler Hemmkonzentration und Hemmhofdurchmesser zeigte, daß mit einem standardisierten Monodisk-Agardiffusionstest die Mezlocillin-, Azlocillin- und Carbenicillin-Empfindlichkeit von Bacteroidaceae-Kulturen nicht hinreichend genau bestimmt werden kann.

Summary

The in vitro susceptibility of approximately 90 Bacteroidaceae strains to the new broad-spectrum penicillins mezlocillin and azlocillin was determined by tube dilution tests and compared to susceptibility to carbenicillin. Bacteroides fragilis and Bacteroides thetaiotaomicron predominated among the strains tested. These strains are particularly important as pathogens and are known to produceβ-lactamase. At concentrations of 16µg/ml or less, mezlocillin inhibited 81% of the Bacteroides cultures, and at 32µg/ml 96% of the cultures. The amount of Bacteroides strains inhibited by 16 and 32µg/ml of azlocillin was 67% and 93% respectively. Similar results were obtained with carbenicillin, concentrations of 32, 64 and 256µg/ml inhibiting 55%, 87% and 94% of the strains respectively. Mezlocillin and azlocillin did not appear to be any more effective than carbenicillin against Sphaerophorus strains. Statistical analysis of the relationship between minimal inhibiting concentration and inhibition zone diameter showed that the susceptibility of Bacteroidaceae to mezlocillin, azlocillin and carbenicillin cannot be determined with sufficient accuracy by a standardized agar diffusion test.

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Authors and Affiliations

  1. Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, Venusberg, D-5300, Bonn 1, Germany

    H. Werner,  C. Krasemann, J. Ungerechts & H. J. Schmitz

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  1. H. Werner
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  2. C. Krasemann
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  3. J. Ungerechts
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  4. H. J. Schmitz
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Werner, H., Krasemann, C., Ungerechts, J. et al. Die In-vitro-Aktivität von Mezlocillin, Azlocillin und Carbenicillin gegen Bacteroidaceae unter besonderer Berücksichtigung der Fragilis-Gruppe. Infection 5, 17–21 (1977). https://doi.org/10.1007/BF01639104

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