Summary
It has been shown, by using the immunogold technique, that C-peptide and insulin are co-localized in the mature granules of human pancreaticβ cells and insulinomas with typical granules. The mean gold bead densities of both C-peptide and insulin were at least twice as high in the normal pancreas when compared with the insulinomas. The mean granule diameter of the insulinoma cells (D=0.30 ±0.12 μm) was smaller than that of human pancreatic cells (D=0.45 ±0.15 μm). The morphometric data indicate that each of the antigens (C-peptide and insulin) is distributed similarly in the halos and the dense cores of theβ granules. Thus, no topological segregation of these two antigens occurs within theβ granules of either normal human pancreas or insulinomas.
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Kalina, M., Grimelius, L., Cedermark, B. et al. Insulin and C-peptide co-localization in theβ granules of normal human pancreas and insulinomas. Vichows Archiv A Pathol Anat 416, 19–23 (1989). https://doi.org/10.1007/BF01606466
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DOI: https://doi.org/10.1007/BF01606466