Skip to main content
SpringerLink
Log in

Different expression and genetic control for the K99 antigen and its associated adhesin

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Cultivation of K99+ wild strains and transconjugants in MINCA medium with variable concentrations of glucose, glycerol, alanine, or protein-interfering antibiotics shows that the K99 antigen and its associated adhesin are expressed in different ways. The addition of cAMP to medium containing a K99-repressive concentration of glucose demonstrates that the K99-antigen production is controlled by the cAMP-CRP complex, but this does not occur with the adhesin production. Sub-inhibitory concentrations of protein-interfering antibiotics inhibit the K99-antigen production, but do not correlatively affect the adhesin production. All these experiments suggest that the K99 antigen and the adhesin are different structures whose genes are controlled by different mechanisms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Curtiss, R. 1981. Gene transfer, pp. 243–256. In: Gerhardt, P. (ed.), Manual of methods for general bacteriology. Washington, DC: American Society for Microbiology.

    Google Scholar 

  2. De Graaf, F. K., Klausen-Boor, P., Van Hees, J. E. 1980. Biosynthesis of the K99 surface antigen is repressed by alanine. Infection and Immunity30:125–128.

    Google Scholar 

  3. De Graaf, F. K., Klemm, P., Gaastra, W. 1981. Purification, characterization and partial covalent structure ofEscherichia coli adhesive antigen K99. Infection and Immunity33:877–883.

    Google Scholar 

  4. Guinée, P. A. M., Veldkamp, J., Jansen, W. H. 1977. Improved MINCA medium for the detection of K99 antigen in calf enterotoxigenic strains ofEscherichia coli. Infection and Immunity15:676–678.

    Google Scholar 

  5. Guinée, P. A. M., Jansen, W. H. 1979. Detection of enterotoxigenicity and attachment factors inEscherichia coli strains of human, porcine and bovine origin: a comparative study. Zentralblatt für Bakteriologie Mikrobiologie und Hygiene243:245–257.

    Google Scholar 

  6. Isaacson, R. E. 1977. K99 surface antigen ofEscherichia coli: purification and partial characterization. Infection and Immunity15:272–279.

    Google Scholar 

  7. Isaacson, R. E. 1978. K99 surface antigen ofEscherichia coli: antigenic characterization. Infection and Immunity22:555–559.

    Google Scholar 

  8. Isaacson, R. E. 1980. Factors affecting expression of theEscherichia coli pilus K99. Infection and Immunity28:190–194.

    Google Scholar 

  9. Morris, J. A., Stevens, A. E., Sojka, W. J. 1977. Preliminary characterization of cell-free K99 antigen isolated fromEscherichia coli B41. Journal of General Microbiology99:353–357.

    Google Scholar 

  10. Morris, J. A., Stevens, A. E., Sojka, W. J. 1978. Anionic and cationic components of the K99 surface antigen fromEscherichia coli B41. Journal of General Microbiology107:173–175.

    Google Scholar 

  11. Morris, J. A., Thorns, C. J., Sojka, W. J. 1980. Evidence for two adhesive antigens on the K99 reference strainEscherichia coli B41. Journal of General Microbiology118:107–113.

    Google Scholar 

  12. Postma, P. W., Roseman, S. 1976. The bacterial phosphoenolpyruvate sugar phosphotransferase system. Biochimica et Biophysica Acta457:213–220.

    Google Scholar 

  13. Silhavy, T. J., Ferenci, T., Boos, W. 1978. Bacterial transport. B. P. Rosen, ed. New York: Marcel Dekker.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departmento de Microbiología, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain

    Carlos M. Ferreiros & Maria-Teresa Criado

Authors
  1. Carlos M. Ferreiros
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria-Teresa Criado
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ferreiros, C.M., Criado, MT. Different expression and genetic control for the K99 antigen and its associated adhesin. Current Microbiology 8, 221–224 (1983). https://doi.org/10.1007/BF01579550

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01579550

Keywords

Search

Navigation