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Osmosensitivity of the 2H+−K+-exchange and the H+-ATPase complex F0F1 in anaerobically grownEscherichia coli

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Abstract

Escherichia coli grown anaerobically for osmotic studies upon increased osmolarity in alkaline medium carried out H+−K+-exchange in two steps, the first of which was DCCD1 sensitive and osmo-dependent and had the 2H+/K+ stoichiometry. H+-efflux in the presence of protonophore (CCCP) upon increase of osmolarity was shown to be high and inhibited by DCCD, whereas H+-efflux induced by a decrease of osmolarity was small and not inhibited by DCCD. The 2H+/K+-exchange was absent intrkA anduncA mutants. InuncB mutant 2H+/K+-exchange was not DCCD-and osmosensitive. Competition between DCCD and osmoshock on inhibition of 2H+/K+-exchange was found. Osmosensitivity of this exchange disappeared in spheroplasts. Osmosensitivity of both 2H+/K+-exchange and the F0F1 and osmoregulation of the F0F1 via F0 and a periplasmic space are postulated.

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Abbreviations

F0F1 :

H+-ATPase complex

F0 :

H+-channel, proteolipid

F1 :

H+-ATPase

Trk :

constitutive system for K+ uptake

PV:

periplasmic protein valve

DCCD:

N,N′-dicyclohexylcarbodiimide

CCCP:

carbonylcyanide-m-chlorophenylhydrazone

ΔμH or ΔμK :

transmembrane electrochemical gradient for H+ or K+ respectively

ΔΦ:

membrane potential

upshock or downshock:

increase or decrease of medium osmolarity, respectively

CGSC:

E. coli Genetic Stock Center, Yale University, USA

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Authors and Affiliations

  1. Department of Biophysics, Biological Faculty of Yerevan State University, 375049, Yerevan, Republic of Armenia

    Armen A. Trchounian, Elena S. Ogandjanian & Polina A. Vanian

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  1. Armen A. Trchounian
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  2. Elena S. Ogandjanian
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  3. Polina A. Vanian
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Trchounian, A.A., Ogandjanian, E.S. & Vanian, P.A. Osmosensitivity of the 2H+−K+-exchange and the H+-ATPase complex F0F1 in anaerobically grownEscherichia coli . Current Microbiology 29, 187–191 (1994). https://doi.org/10.1007/BF01570152

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