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Nonspecific binding of mouse monoclonal antibodies toClostridium difficile toxins A and B

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Abstract

Several recent reports have described large numbers of monoclonal antibodies that cross-react with toxins A and B ofClostridium difficile; this suggests that the toxins share major epitopes. Our results show that monoclonal antibodies (MAb) against other antigens bind nonspecifically to both toxins. Therefore, we believe that the cross-reacting MAb bind by this manner and not by a true immune reaction.

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Literature Cited

  1. Banno Y, Kobayashi T, Kono H, Watanabe K, Ueno K, Nozawa Y (1984) Biochemical characterization and biologic actions of two toxins (D-1 and D-2) fromClostridium difficile. Rev Infect Dis 6:S11-S20

    PubMed  Google Scholar 

  2. Eichel-Streiber C, Harperath U, Bosse D, Hadding U (1987) Purification of two high molecular weight toxins ofClostridium difficile which are antigenically related. Microb Pathogen 2:307–318

    Google Scholar 

  3. Laemmli U (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    PubMed  Google Scholar 

  4. Lyerly D, Phelps C, Toth J, Wilkins T (1986) Characterization of toxins A and B ofClostridium difficile with monoclonal antibodies. Infect Immun 54:70–76

    PubMed  Google Scholar 

  5. Lyerly D, Roberts M, Phelps C, Wilkins T (1986) Properties of toxins A and B ofClostridium difficile. FEMS Microbiol Lett 33:31–35

    Google Scholar 

  6. Lyerly D, Krivan H, Wilkins T (1988)Clostridium difficile: its disease and toxins. Clin Microbiol Rev 1:1–18

    PubMed  Google Scholar 

  7. Rothman S, Gentry M, Brown J, Foret D, Stone M, Strickler M (1988) Immunochemical and structural similarities in toxin A and toxin B ofClostridium difficile shown by binding to monoclonal antibodies. Toxicon 26:583–597

    PubMed  Google Scholar 

  8. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc Natl Acad Sci USA 76:4350–4354

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA

    David M. Lyerly, Pauline E. Carrig & Tracy D. Wilkins

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  1. David M. Lyerly
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  2. Pauline E. Carrig
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  3. Tracy D. Wilkins
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Lyerly, D.M., Carrig, P.E. & Wilkins, T.D. Nonspecific binding of mouse monoclonal antibodies toClostridium difficile toxins A and B. Current Microbiology 19, 303–306 (1989). https://doi.org/10.1007/BF01570105

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  • DOI: https://doi.org/10.1007/BF01570105

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