Abstract
Growth ofEubacterium limosum B2 on methanol-CO2 was dependent upon the pH; optimum growth rates were obtained at pH 7.3–7.4. Carbon dioxide, a necessary cosubstrate for methylotrophic growth on methanol, was a determining factor for both growth and the nature of the organic acid produced. The coefficient of affinity for CO2 was not affected by variations in pH if calculated relative to the concentration of hydrogen carbonate (16 mM). Similarly the conversion rate for methanol into acetic acid and butyric acid remained constant regardless of the level of pH with a fixed concentration of hydrogen carbonate. The metabolism ofE. limosum B2 grown on methanol-CO2 was regulated by the HCO -3 concentration in the medium.
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Pacaud, S., Loubiere, P. & Goma, G. Methanol metabolism byEubacterium limosum B2: Effects of pH and carbon dioxide on growth and organic acid production. Current Microbiology 12, 245–250 (1985). https://doi.org/10.1007/BF01567972
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DOI: https://doi.org/10.1007/BF01567972