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Vibrio ordalii sp. nov.: A causative agent of vibriosis in fish

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Abstract

Vibrio ordalii sp. nov. is the name proposed for the bacterium previously designated asV. anguillarum biotype 2. The change in the classification of this fish pathogen is based on differences between the classicalV. anguillarum andV. ordalii in cultural and biochemical characteristics, and in deoxyribonucleic acid (DNA) sequence relatedness. Phenotypically,V. ordalii was distinguishable fromV. anguillarum based on: negative Voges-Proskauer reaction; negative reaction with arginine in Moeller's medium; negative Simmons' and Christensen's citrate test; negative ONPG test; failure to hydrolyze starch; failure to show lipase activity; inability to grow at 37°C; and failure to ferment cellobiose, glycerol, sorbitol, and trehalose. Genotypically, strain ofV. ordalii formed a highly conserved DNA homology group which showed 83 to 100% within-group homology and only 58 to 69% relatedness toV. anguillarum. In contrast, theV. anguillarum strains tested showed greater than 70% withingroup homology and 53 to 67% relatedness toV. ordalii. NeitherV. ordalii norV. anguillarum were related toV. parahaemolyticus orV. alginolyticus. The proposed type strain (holotype) ofV. ordalii is ATCC 33509 (=DF3K=Dom F3 kid).

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Authors and Affiliations

  1. Environmental Conservation Division, Northwest and Alaska Fisheries Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 2725 Montlake Boulevard East, 98112, Seattle, Washington, USA

    Michael H. Schiewe

  2. Department of Biochemistry and Microbiology, University of Victoria, V8W 2Y2, Victoria, British Columbia, Canada

    Trevor J. Trust

  3. Department of Microbiology and Immunology, University of Oregon Medical School, 3181 S. W. Sam Jackson Park Road, 97201, Portland, Oregon, USA

    Jorge H. Crosa

Authors
  1. Michael H. Schiewe
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  2. Trevor J. Trust
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  3. Jorge H. Crosa
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Schiewe, M.H., Trust, T.J. & Crosa, J.H. Vibrio ordalii sp. nov.: A causative agent of vibriosis in fish. Current Microbiology 6, 343–348 (1981). https://doi.org/10.1007/BF01567009

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