Abstract
Secondary mixed lymphocyte reaction (MLR-II) was studied in A.TH anti A.TL and A.TL anti-A.TH combinations in which stimulation was mainly due toH-2I-region differences. In both cases the MLR-II was specifically inhibited by the responder anti-stimulator Ia serum. The level of inhibition was dependent on the ratio of the amount of immune serum to the number of stimulating cells. The inhibitory activity and Ia antibodies were specifically absorbed and eluted together. The results confirm that the lymphocyte-activating determinants of the MLR-II (1) are carried by the Ia molecules and (2) are identical to the serologically defined Ia determinants. —Anti-Ia sera directed against private and public specificities of the stimulating cell induced a higher level of inhibition than anti-Ia sera directed only against public specificities, indicating that both private and public Ia specificities are involved in restimulation during MLR-II. — These results, in connection with others, suggest that the receptor of the proliferating T cell recognizes the same Ia determinant as the combining site of the Ia-recognizing antibody.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- Lad:
-
lymphocyte-activating determinant
- MLR:
-
mixed lymphocyte reaction
- MLR-I, MLR-II:
-
primary, secondary MLR
- PRC:
-
primed responder cells
- LCT:
-
dye exclusion lymphocytotoxicity microtechnique
- RR:
-
relative response
References
Abassi, K., Demant, P., Festenstein, H., Holmes, J., Huber, B., and Rychlikova, M.: Mouse mixed lymphocyte reactions and cell mediated lympholysis: genetic control and relevance to antigenic strength.Transplant. Proc. 5: 1329–1337, 1973
Binz, H. and Wigzell, H.: Antigen binding, idiotypic T-lymphocyte receptors.Contemp. Top. Immunobiol. 1: 113–177, 1977
Binz, H., Frischknecht, X., Shen, F. W., and Wigzell, H.: Idiotypic determinants on T-cell sub-populations.J. exp. Med. 149: 910–922, 1979
Colombani, J., Colombani, M., Shreffler, D. S., and David, C. S.: Separation of anti-Ia (I-region associated antigens) from anti-H-2 antibodies in complex sera, by absorption on blood platelets. Description of three new Ia specificities.Tissue Antigens 7: 74–85, 1976
David, C. S., Beisel, K. W., Krco, C. J., and Lafuse, W. P.: Genetic organization, tissue expression and functional role of murine Ia antigens.In R. E. Reisfeld (ed.):Current Trends in Histocompatibility. Plenum Press, New York, in press 1980
Fathman, C. G., Collavo, D., Davies, S., and Nabholz, M: In vitro secondary MLR. I. Kinetics of proliferation and specificity of in vitro primed responder cells.J. Immunol. 118: 1232–1238, 1977
Frelinger, J. A., Niederheber, J. E., David, C. S., and Shreffler, D. C.: Evidence for the expression of Ia (H-2 associated) antigens on thymus derived lymphocytes.J. Exp. Med. 140: 1273–1284, 1974
Guimezanes, A., Colombani, M., and Colombani, J.: Secondary mouse mixed lymphocyte reaction (MLR) in vitro: correlation between primed cells reactivity and serological typing for Ia specificities.Immunogenetics 8: 127–137, 1979
Julius, M. M., Simpson, E., and Herzenberg, L. H.: A rapid method for the isolation of functional thymus-derived murine lymphocytes.Eur. J. Immunol 3: 645–649, 1973
Klein, J., Flaherty, L., VandeBerg, J. L., and Shreffler, D. C.:H-2 haplotypes, genes, regions and antigens: first listing.Immunogenetics 6: 489–512, 1978
Lafuse, W. P., McCormick, J. F., and David, C. S.: Serological and biochemical identification of hybrid Ia antigens.J. Exp. Med. 151: 709–715, 1980
Meo, T., David, C. S., Rijnbeek, A. M., Nabholz, M., Miggiano, V. C., and Shreffler, D. C.: Inhibition of mouse MLR by anti-Ia sera.Transplant. Proc. 7: 127–129, 1975
Nagy, Z., Elliott, B. E., Nabholz, M., Drammer, P. H., and Pernis, B.: Specific binding of alloantigens to T cells activated in the mixed lymphocyte reaction.J. Exp. Med 143: 648–659, 1976 a
Nagy, Z., Elliott, B. E., and Nabholz, M.: Specific binding of K- and I-region products of the H-2 complex to activated thymus-derived (T) cells belonging to different Ly subclasses.J. Exp. Med. 144: 1545–1553, 1976b
Peck, A. B. and Bach, F. H.: A miniaturized mouse mixed leucocyte culture in serum free and mouse serum supplemented media.J. Immunol. Methods 3: 147–163, 1973
Peck, A. B., Wigzell, H., Janeway, C., and Andersson, L. C.: Environmental and genetic control of T-cell activation in vitro: a study using isolated alloantigens-activated T-cell clone.Immunol. Rev. 35: 146–180, 1977
Peck, A. B. and Wigzell, H.: The mouse primed lymphocyte typing (mPLT) test. I. Definition of the SD and Ia molecules as H-2-associated lymphocyte-stimulating (LS) determinants.Immunogenetics 6: 529–545, 1978
Pla, M. and Colombani, J. M.: Etude des déterminants responsables de Ia stimulation lors de la reaction lymphocytaire mixte secondaire.C. R. Acad. Sci. (D) (Paris)289: 477–480, 1979
Pla, M., Birnbaum, D., Guimezanes, A., Colombani, M., and Colombani, J.: Correlation between serological and cellular methods for the definition of Ia specificities.Transplant Proc., in press 1980
Schwartz, R. H., Fathman, C. G., and Sachs, D. H.: Inhibition of stimulation in murine mixed lymphocyte cultures with an alloantiserum directed against a shared Ia determinant.J. Immunol. 116: 929–935, 1976
Shreffler, D. C., Amos, D. B., and Mark, R.: Serological analysis of a recombination in the H-2 region of the mouse.Transplantation 4: 300–322, 1966
Shreffler, D. C. and David, C. S.: The H-2 major histocompatibility complex and I immune response region: genetic variation, function and organization.Adv. Immunol. 20: 125–195, 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pla, M., Colombani, M. & Colombani, J. Inhibition of secondary mouse mixed lymphocyte reaction by anti-Ia sera. Immunogenetics 12, 285–295 (1981). https://doi.org/10.1007/BF01561671
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01561671