Abstract
Five H-2 and seven Ia monoclonal antibodies were tested against a panel of 43 independentH-2 haplotypes (11 of laboratory-mouse and 32 of wild-mouse origin), 33 recombinantH-2 haplotypes, and up to 74 wild mice. All the antibodies gave negative reactions in the PVP hemagglutination tests; all, however, gave positive reaction with some members of the panel in the dye-exclusion cytotoxic test. Four of the antibodies (H-2.m2,Ia.m2, Ia.m5 and Ia.m7) reacted identically to conventional antibodies detecting determinants H-2.2., and Ia-1.2, Ia-1.15, and Ia-5.7, respectively (this statement does not apply to wild mice in which minor differences in reactivity patterns of the corresponding antibodies were found: the reproducibility of these differences, however, could not be checked by absorption). Five other antibodies (H-2.m5, H-2.m3, H-2.m4, Ia.ml, and Ia.m6) had very similar though not identical reactivity patterns to conventional antibodies detecting determinants H-2.5, H-2.11, H-2.25, Ia-1.2, and Ia-1.19, respectively. The last three monoclonal antibodies (H-2.ml, Ia.m3, and Ia.m4) had a reactivity pattern that did not match those of any known conventional antibodies. The near identity or great similarity of many monoclonal and conventional antibodies indicates that the “cleanest” of the conventional antisera are truly monospecific, and gives credence to the H-2 serology as defined by conventional antibodies. The serological analysis of monoclonal antibodies supports the true existence of private and public determinants, and reveals that the H-2 and Ia determinants are complex, even when the antibody is simple.
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Hämmerling, G. J., Lemke, H., Hämmerling, U., Höhmann, C., Wallich, R., and Rajewsky, K.: Monoclonal antibodies against murine cell surface antigens: Anti-H-2, anti-Ia and anti-T cell antibodies.Curr. Top. Microbiol. Immunol. 81:100–106, 1978
Hämmerling, G. J., Hämmerling, U., Lemke, H. and Rajewsky, K.: Isolation of twelve monoclonal antibodies against Ia and H-2 antigens. Serological characterization and reactivity with B and T lymphocytes.Immunogenetics 8: 1979
Hirschfeld, J.: Serologic codes: Interpretation of immunogenetic system.Science 148:968–970, 1965
Klein, J.:Biology of the Mouse Histocompatibility-2 Complex. Springer-Verlag, New York, 1975
Klein, J. and Shreffler, D. C.: The H-2 model for major histocompatibility systems.Transplant. Rev. 6: 3–29, 1971
Klein, J., Hauptfeld, V., and Hauptfeld, M.: Evidence for a fifth (G) region in theH-2 complex of the mouse.Immunogenetics 2:141–150, 1975
Klein, J., Flaherty, L., VandeBerg, J. L., and Shreffler, D. C.:H-2 haplotypes, genes, regions, and antigens: First listing.Immunogenetics 6:489–512, 1978
Köhler, G. and Milstein, D.: Continuous cultures of fused cells secreting antibody of predefined specificity.Nature 256:495–497, 1975
Lemke, H., Hämmerling, G. J., Höhmann, D., and Rajewsky, K.: Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse.Nature 271:249–251, 1978
Melchers, F., Potter, J., and Warner, N. (eds.):Lymphocyte Hybridomas. Curr. Top. Microbiol. Immunol. Vol. 81. Springer-Verlag, Berlin 1978
Oi, V. T., Jones, P. P., Goding, J. W., and Herzenberg, L. A.: Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens.Curr. Top. Microbiol. Immunol. 81:115–128, 1978
Snell, G. D., Cherry, M., and Démant, P.: H-2: Its structure and similarity to HL-A.Transplant. Rev. 15:3–25, 1973
Stimpfling, J. H.: The use of PVP as a developing agent in mouse hemagglutination test.Transplant. Bull. 27:109–111, 1961
Thorsby, E.: A tentative new model for the organization of the mouse H-2 histocompatibility system. Two segregant series of antigens.Eur. J. Immunol. 1:57–59, 1971
Wakeland, E. K. and Klein, J.: Histocompatibility-2 system in wild mice. VII. Serological analysis of 29 wild-derivedH-2 haplotypes using antisera to inbred I-region antigens.Immunogenetics 8;27–39, 1979
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Klein, J., Huang, H.J.S., Lemke, H. et al. Serological analysis of H-2 and Ia molecules with monoclonal antibodies. Immunogenetics 8, 419–432 (1979). https://doi.org/10.1007/BF01561453
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DOI: https://doi.org/10.1007/BF01561453