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Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase

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Somatic Cell and Molecular Genetics

Abstract

The genes for the serine proteases trypsin, chymotrypsin B, and elastase were chromosomally assigned in man using cDNA probes that have been isolated from a rat pancreatic cDNA library. DNA from human × rodent somatic cell hybrids was cleaved with BamHI or EcoRI and analyzed by Southern filter hybridization methods for the segregation of the genes for trypsin-1 (TRY1), chymotrypsin B (CTRB), and elastase-1 (ELA1). TRY1 was assigned to human chromosome 7q22→qter, CTRB to chromosome 16, and ELA1 to chromosome 12. Although the three genes are members of the same gene family, they are dispersed over different chromosomes.

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Author notes

  1. N. K. Honey

    Present address: Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand

  2. C. Quinto

    Present address: Centro de Figacion de Nitrogeno, Cuernavaca, Mexico

  3. G. I. Bell

    Present address: Chiron Corporation, Emeryville, California

  4. S. L. Naylor

    Present address: Department of Cellular and Structural Biology, University of Texas Health Science Center, 78284, San Antonio, Texas

Authors and Affiliations

  1. Roswell Park Memorial Institute, 14263, Buffalo, New York

    N. K. Honey, A. Y. Sakaguchi & S. L. Naylor

  2. University of California, San Francisco, California

    C. Quinto, G. I. Bell, C. Craik & W. J. Rutter

  3. University of Texas Health Science Center at Dallas, Texas

    R. J. MacDonald

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  1. N. K. Honey
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  2. A. Y. Sakaguchi
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  3. C. Quinto
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  4. R. J. MacDonald
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  6. C. Craik
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  7. W. J. Rutter
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  8. S. L. Naylor
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Additional information

Preliminary chromosome assignments of chymotrypsin B (1) and of trypsin and elastase (2) have been made in abstract.

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Honey, N.K., Sakaguchi, A.Y., Quinto, C. et al. Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase. Somat Cell Mol Genet 10, 369–376 (1984). https://doi.org/10.1007/BF01535632

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  • DOI: https://doi.org/10.1007/BF01535632

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