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Irradiation-reduced human chromosome 21 hybrids

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Somatic Cell and Molecular Genetics

Abstract

Rodent-human somatic cell hybrids have been constructed which contain fragments of human chromosome 21 as their only human material. This was done by irradiating rodent-human somatic cell hybrids containing a complete chromosome 21 to fragment the genome and then rescuing human GAR synthetase and various amounts of flanking chromosome 21 DNA by fusing with GAR synthetase-deficient hamster cells and selecting for growth in purine-free medium. Four irradiation-reduction hybrids were produced by this method and contain the distal, proximal, and central portions of the long arm of human chromosome 21, all centered about GAR synthetase. These irradiation-reduction hybrids were used as a panel to regionally map single-copy and individual copies of repetitive sequences. Using these hybrids along with another independently constructed hybrid, the GAR synthetase gene was mapped distal to SOD-1 and proximal to CP21G1(D21S60). Of special interest is the regional mapping of the gene for the amyloid β-protein distal to pPW236B(D21S11) and proximal to SOD-1.

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Literature cited

  1. Kao, FT. (1983).Int. Rev. Cytol. 85:109–145.

    PubMed  Google Scholar 

  2. Van Keuren, M.L., Watkins, P.C., Drabkin, H.A., Jabs, E.W., Gusella, J.F., Patterson, D. (1986).Am. J. Hum. Genet. 38:793–804.

    PubMed  Google Scholar 

  3. Goss, S.J., Harris, H. (1977).J. Cell. Sci. 25:17–37.

    PubMed  Google Scholar 

  4. Goss, S.J., Harris, H. (1977).J. Cell Sci. 25:39–57.

    PubMed  Google Scholar 

  5. Cirullo, R.E., Dana, S., Wasmuth (1983).J. Mol. Cell Biol,3:892–902.

    Google Scholar 

  6. Tanzi, R.E., Gusella, J.F., Watkins, P.C., Bruns, G.A.P., St. George-Hyslop, P., Van Keuren, M.L., Patterson, D., Pagan, S., Kurnit, D.M., Neve, R.L. (1987).Science 235:880–884.

    PubMed  Google Scholar 

  7. Ham, R.G. (1965).Proc. Natl. Acad. Sci. U.S.A. 53:288–293.

    PubMed  Google Scholar 

  8. Kao, F.-T., Puck, T.T. (1974). InMethods in Cell Biology, 8th ed., (Ed.) D.M. Prescott, (Academic Press, New York), pp. 23–39.

    Google Scholar 

  9. Patterson, D., Kao, F.-T., Puck, T.T. (1974).Proc. Natl. Acad. Sci. U.S.A. 71:2057–2061.

    PubMed  Google Scholar 

  10. Irwin, M., Oates, D.C., Patterson, D. (1977).Somat. Cell Genet. 5:203–216.

    Google Scholar 

  11. Moore, E.E., Jones, C., Kao, F.-T., Oates, D.C. (1977).Am. J. Hum. Genet. 29:389–396.

    PubMed  Google Scholar 

  12. Patterson, D., Graw, S., Jones, C..Proc. Natl. Acad. Sci. U.S.A. 78:405–409, 1981.

    PubMed  Google Scholar 

  13. Miller, Y.E., Jones, C.A., Scoggin, C.H., Patterson, D.Trisomy 21 (in press).

  14. Harris, H., Watkins, J., Ford, C.E., Schoefl, G. (1966).J. Cell Sci. 1:1.

    PubMed  Google Scholar 

  15. Morse, H.G., Patterson, D., Jones, C. (1982).Mammal. Chromosomes Newslett. 23:126–133.

    Google Scholar 

  16. Harper, M., Saunders, G.F. (1981).Chromosoma 83:431–439.

    PubMed  Google Scholar 

  17. Rigby, B.W.J., Dieckmann, M., Rhodes, C., Berg, P. (1977).J. Mol. Biol. 113:237–251.

    PubMed  Google Scholar 

  18. Oates, D.C., Patterson, D. (1977).Somat. Cell Genet. 3:561–577.

    PubMed  Google Scholar 

  19. Patterson, D., Schandle, V.B. (1983). Banbury Report 14: Recombinant DNA Applications to Human Disease (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).

    Google Scholar 

  20. Meera Khan, P. (1971).Arch. Biochem. Biophys. 145:470–483.

    PubMed  Google Scholar 

  21. Gusella, J., Varsanyi-Breiner, A., Kao, F.-T., Jones, C., Puck, T.T., Kays, C., Orkin, S., Housman, D. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:5239–5243.

    PubMed  Google Scholar 

  22. Maniatis, T., Fritsch, E.F., Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).

    Google Scholar 

  23. Southern, E.M. (1975).J. Mol. Biol. 98:503–517.

    PubMed  Google Scholar 

  24. Tan, Y.H., Tischfield, J., Ruddle, F.H. (1973).J. Exp. Med. 137:317–330.

    PubMed  Google Scholar 

  25. Levanon, D., Leiman-Hurwitz, J., Dafni, N., Wigderson, M., Sherman, L., Bernstein, Y., Laver-Rudick, Z., Danciger, E., Stein, O., Groner, Y. (1985).EMBO J. 4:77–84.

    PubMed  Google Scholar 

  26. Gusella, J.F., Tanzi, R.E., Watkins, P.C., Gibbons, K.T., Hobbs, W.J., Faryniarz, A.G., Healy, S.T., Anderson, M.A. (1985).Ann. N.Y. Acad. Sci. 450:25–31.

    PubMed  Google Scholar 

  27. Patterson, D., Graw, S., Gusella, J., Watkins, P. (1987). In,New Perspectives on Down Syndrome, (eds.) Pueschel S.M., Tingey C., Rynders, J., Crocker, A., and Crutcher, D.M. (Paul Brookes Pub. Co.), pp. 47–68.

  28. Hards, R.G., Benkovic, S.J., Van Keuren, M.L., Graw, S.L., Drabkin, H.A., Patterson, D. (1986).Am. J. Hum. Genet. 37:179–185.

    Google Scholar 

  29. Henikoff, S., Keene, M.A., Sloan, J.S., Bleskan, J., Hards, R., Patterson, D. (1986).Proc. Natl. Acad. Sci. U.S.A. 83:720–724.

    PubMed  Google Scholar 

  30. Davidson, J.N., Davidson, A.A., Niswander, L.A. (1984). InAdvances in Gene Technology: Human Genetic Disorders. Proceedings of the 16th Miami Winter Symposium, Miami, Fl (ICSN Press), pp. 148–149.

    Google Scholar 

  31. Davidson, J.N., Rumsby, G., Niswander, L.A. (1985).Ann. N.Y. Acad. Sci. 450:43–54.

    PubMed  Google Scholar 

  32. Gusella, J.F., Jones, C., Kao, F. -T., Housman, D., Puck, T.T. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:7804–7808.

    PubMed  Google Scholar 

  33. Duncan, C., Biro, P.A., Choudary, P.V., Elder, J.T., Wang, R.R.C., Forget, B.G., de Riel, J.K., Weissman, S.M. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:5095–5098.

    PubMed  Google Scholar 

  34. Callahan, R., Drohan, W., Tronick, S., Schlom, J.,Proc. Natl. Acad. Sci. U.S.A. (1982).79:5503–5507.

    PubMed  Google Scholar 

  35. Chadefaux, B., Allard, O., Rethore, M.O., Raoul, O., Poissonier, M., Gilgenkrantz, S., Vhervy, C., Jerome, H. (1984).Hum. Genet. 66:190–192.

    PubMed  Google Scholar 

  36. Smith, G.F., Warren, S.T. (1985).Ann. N.Y. Acad. Sci. 450:1–9.

    Google Scholar 

  37. Schwartz, D.C., Cantor, C. (1984).Cell 37:67–75.

    PubMed  Google Scholar 

  38. Gardiner, K., Laas, W., Patterson, D. (1986).Somat. Cell Molec. Genet. 12:185–195.

    PubMed  Google Scholar 

  39. Neibuhr, E. (1974).Humangenetik 21:99–101.

    PubMed  Google Scholar 

  40. Hoehn, H., (1975).Am. J. Hum. Genet. 27:676–686.

    PubMed  Google Scholar 

  41. Miklos, G.L.G., John, B. (1979).Am. J. Hum. Genet. 31:264–280.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts

    Sharon Graw

  2. University of Kentucky, 40636, Lexington, Kentucky

    Jeffrey Davidson

  3. Harvard Medical School, 02114, Boston, Massachusetts

    James Gusella & Rudolph Tanzi

  4. Integrated Genetics, 01701, Framingham, Massachusetts

    Paul Watkins

  5. Children's Hospital, 02115, Boston, Massachusetts

    Rachael Neve

  6. Eleanor Roosevelt Institute for Cancer Research, 80206, Denver, Colorado

    David Patterson

  7. University of Colorado Health Sciences Center, 80262, Denver, Colorado

    Sharon Graw & David Patterson

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  1. Sharon Graw
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  2. Jeffrey Davidson
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  3. James Gusella
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  4. Paul Watkins
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  5. Rudolph Tanzi
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  6. Rachael Neve
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  7. David Patterson
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Graw, S., Davidson, J., Gusella, J. et al. Irradiation-reduced human chromosome 21 hybrids. Somat Cell Mol Genet 14, 233–242 (1988). https://doi.org/10.1007/BF01534584

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  • DOI: https://doi.org/10.1007/BF01534584

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