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Combined activation of murine lymphocytes with staphylococcal enterotoxin and interleukin-2 results in additive cytotoxic activity

  • Ogininal Articles
  • Lymphokine-activated Killer Cell, Cytotoxic Lymphocytes, Staphylococcal-enterotoxin-dependent Cell-mediated Cytotoxicity
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Abstract

This report demonstrates that in vitro activation of murine spleen cells with interleukin-2 (IL-2) or the bacterial superantigen staphylococcal enterotoxin A (SEA) results in different patterns of activation and function of cytotoxic cells. Lymphokine-activated killer activity and antibody-dependent cellular cytotoxicity (ADCC) are mainly mediated by IL-2 activated natural killer (NK) cells. SEA is the most powerful T cell mitogen known so far and retarets cytotoxic T lymphocytes (CTL) to tumors expressing major histocompatibility complex (MHC) class II in staphylococcal-enterotoxin-dependent cellular cytotoxicity (SDCC). Culture of mouse spleen cells with SEA led to expansion and activation of T cells which demonstrated strong SDCC activity and some NK-like cytotoxicity after 5 days in culture. Cell sorting revealed that both CD8+ and CD4+ T cells mediated SDCC but the former were more effective. Phenotypic analysis showed that SEA preferentially stimulated and expanded T cells expressing T cell receptor Vβ11, in particular CD8+ T cells. Combined activation with SEA and IL-2 resulted in simultaneous induction of T and NK cell cytotoxicity. Moreover, IL-2 had additive effects on SEA-induced SDCC. Combined treatment with SEA and IL-2 might therefore be an approach to induce maximal cytotoxicity against tumors and to recruit both T and NK cells in tumor therapy.

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Authors and Affiliations

  1. Department of Tumor Immunology, The Wallenberg Laboratory, University of Lund, Sölvegatan 33, 220 07, Lund, Sweden

    Hans Belfrage, Pallonji Bhiladvala, Gunnar Hedlund, Mikael Dohlsten & Terje Kalland

  2. Kabi Pharmacia Oncology, Lund, Sweden

    Gunnar Hedlund, Mikael Dohlsten & Terje Kalland

Authors
  1. Hans Belfrage
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  2. Pallonji Bhiladvala
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  3. Gunnar Hedlund
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  4. Mikael Dohlsten
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  5. Terje Kalland
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Belfrage, H., Bhiladvala, P., Hedlund, G. et al. Combined activation of murine lymphocytes with staphylococcal enterotoxin and interleukin-2 results in additive cytotoxic activity. Cancer Immunol Immunother 38, 265–271 (1994). https://doi.org/10.1007/BF01533518

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  • DOI: https://doi.org/10.1007/BF01533518

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