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Antibodies to tumor necrosis factor: a component of B cell immune responses with a role in tumor/host interaction

  • Original Article
  • Tumor-Infiltrating Lymphocytes, B Lymphocytes, Tumor Necrosis Factor, TNF
  • Published:
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Abstract

Infiltrating B lymphocytes are found within tumors, where their role and the antigens they recognize are poorly defined. After in vitro expansion of these cells, we were able to detect the production of antibodies to tumor necrosis factor α (TNF) in 13 of 17 human tumors studied. These antibodies were detected by both enzyme-linked immunosorbent assay and by neutralization. Anti-TNF antibodies were not produced by resting peripheral blood B cells of normal subjects. However, anti-TNF antibodies were produced by B cells obtained from healthy individuals, after either in vivo or in vitro antigenic stimulation. This suggests that anti-TNF antibody production may constitute part of the overall B cell response to antigens. The intratumoral production of anti-TNF antibody may play a role in tumor/host interactions.

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Author notes

  1. José Alexandre M. Barbuto

    Present address: Visiting Scientist at the Arizona Cancer from the Depto de Immunologia, ICB-USP, Brazil

Authors and Affiliations

  1. Visiting Scientist at the Arizona Cancer from the Depto de Immunologia, ICB-USP, Brazil

    William J. Grimes & Evan M. Hersh

Authors
  1. José Alexandre M. Barbuto
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  2. William J. Grimes
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  3. Evan M. Hersh
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Additional information

This work was supported by grants from FAPESP (90/1844-4) and from the Share Foundation and the Concern Foundation

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Barbuto, J.A.M., Grimes, W.J. & Hersh, E.M. Antibodies to tumor necrosis factor: a component of B cell immune responses with a role in tumor/host interaction. Cancer Immunol Immunother 40, 31–36 (1995). https://doi.org/10.1007/BF01517233

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  • DOI: https://doi.org/10.1007/BF01517233

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