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Dorsal root repair by means of an autologous nerve graft: Experimental study in the rat

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Summary

Rat dorsal root regeneration was studied after 6th and 7th cervical root surgical removal and replacement with an autologous graft of peripheral nerve harvested from the surval nerve from dorsal root ganglion to dorsal horn. Histological studies showed axonal regeneration within the grafts. When the distal end of the graft was placed inside the posterior horn of the spinal cord by use of a myelotomy, axonal sprouts (revealed by the transganglionic staining method of horseradish peroxidase or HSP) reached the neurones of the posterior horn in a limited fashion.

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References

  1. Aguayo AJ, Bray GM (1980) Experimental nerve grafts. In: Jewett DL, McCorroll HR Jr (eds) Nerve repair and regeneration. Its clinical and experimental basis. CV Mosby, St Louis, pp 68–76

    Google Scholar 

  2. Aguayo AJ, Benfey M, David S (1983) A potential for axonal regeneration in neurons of the adult mammalian nervous system. In: Guiffrida-Stella A, Perez-Polo R, Haber B (eds) Birth defects, vol 9, chapter 4. Liss, New York, pp 327–340

    Google Scholar 

  3. Aguayo AJ, David, S, Bray GM (1981) Influences of the glial environment on the elongation of axons after injury: Transplantation studies in adult rodents. J Exp Biol 95: 231–240

    PubMed  Google Scholar 

  4. Aguayo AJ, David S, Richardson P, Bray G (1982) Axonal elongation in peripheral and central nervous system transplants. In: Federoff S, Hertz L (eds) Advances in cellular neurobiology, vol 3. Academic Press, New York, pp 215–234

    Google Scholar 

  5. Aguayo AJ, Dickson R, Trecarten J, Attiwell M., Bray GM (1978) Ensheathment and myelination of regenerating PNS fibres by transplanted optic nerve glia. Neurosci Lett 9: 97–104

    Google Scholar 

  6. Aguayo AJ, Kasarjian J, Skamene E (1977) Myelination of mouse axons by Schwann cells transplanted from normal and abnormal human nerves. Nature 268: 753–755

    PubMed  Google Scholar 

  7. Aguayo AJ, Richardson PM, David S, Benefey M (1982) Transplantation of neurons and sheath cells. A tool for the study of regeneration. In: Nichols GJP (ed) Repair and regeneration of the nervous system. Springer, Berlin Heidelberg New York, pp 91–105

    Google Scholar 

  8. Aguayo AJ (1985) Axonal regeneration from injured neurons in the adult mammalian central nervous system. In: Cotman CW (ed) Synaptic plasticity. Guilford, New York, pp 457–484

    Google Scholar 

  9. Bonney G (1977) Some lesions of the brachial plexus. Ann R Coll Surg Engl 59: 298–306

    PubMed  Google Scholar 

  10. Bonney G (1991) Nerve root, plexus and peripheral nerve trauma. In: Suxesh M, Oxbury J (eds) Clinical neurology. Churchill Livingstone, Edinburg, pp 738–766

    Google Scholar 

  11. Carlsson CA, Sundin T (1968) Reconstruction of severed ventral roots innervating the urinary bladder. Scand J Urol Nephrol 2: 199–210

    PubMed  Google Scholar 

  12. Carlstedt T (1977) Observations on the morphology and the transition between the peripheral and the central nervous system in the cat. I. A preparative procedure useful for electron microscopy of the lumbosacral dorsal rootlets. Acta Physiol Scand [Suppl] 446: 5–18

    Google Scholar 

  13. Carlstedt T (1977) Observations on the morphology and the transition between the peripheral and the central nervous system in the cat. IV. Unmyelinated fibers in S1 dorsal rootlets. Acta Physiol Scand [Suppl] 446: 5–18

    Google Scholar 

  14. Carlstedt T (1983) Regrowth of anastomosed ventral root nerve fibres in the dorsal root of rats. Brain Res 272: 162–165

    PubMed  Google Scholar 

  15. Carlstedt T (1985) Regenerating axons from nerve terminals at astrocytes. Brain Res 347: 188–191

    PubMed  Google Scholar 

  16. Carlstedt T (1985) Regrowth of cholinergic and catecholaminergic neurons along a peripheral and central nervous pathway. Neuroscience 15: 507–518

    PubMed  Google Scholar 

  17. Carlstedt T (1985) Dorsal root innervation of spinal cord neurons after dorsal root implantation into the spinal cord of adult rats. Neurosci Lett 74: 343–348

    Google Scholar 

  18. Carlstedt T (1989) Reinnervation of the mammalian spinal cord after neonatal dorsal root crush. J Neurocytol 17: 335–350

    Google Scholar 

  19. Carlstedt T, Dalsgaard CJ, Molander C (1987) Regrowth of lesioned dorsal root nerve fibre into the spinal cord of neonatal rats. Neurosci Lett 84: 14–18

    Google Scholar 

  20. Carlstedt T, Risling H, Lindå H, Cullheim S, Ulfhake B (1990) Regeneration after spinal nerve root injury. Rest Neurol Neurosci 1: 289–295

    Google Scholar 

  21. Carlstedt T, Grane P, Hallin RG, Norén G (1995) Return of function after spinal cord implantation of avulsed spinal nerve roots. Lancet 364: 1323–1325

    Google Scholar 

  22. Chung BS, Sheen K, Chung JM (1991) Evidence of invasion of regenerated ventral root afferents into the spinal cord of the rat subjected to sciatic neurectomy during the neonatal period. Brain Res 552(2): 311–319

    PubMed  Google Scholar 

  23. Contamin F (1976) Recherches sur la répouse intra-cordonale de fibres sensitives sectionées dans la racine postérieure chez le chat. Rev Neurol (Paris) 132(7): 445–466

    Google Scholar 

  24. David S, Aguayo AJ (1981) Axonal elongation into peripheral nervous system “bridges” after central nervous system injury in adult rats. Science 214: 931–933

    PubMed  Google Scholar 

  25. Duce IR, Reves JF, Keen P (1976) A scanning electron microscope study of the development of free axonal sprouts at the cut ends of dorsal spinal nerve roots in the rat. Cell Tiss Res 170: 507–513

    Google Scholar 

  26. Frazier CH, Mills C (1912) Intradural root anastomosis for the relief of paralysis of the bladder. J Am Med Ass 59: 2200–2206

    Google Scholar 

  27. Freeman LW (1952) Observations on spinal nerve root transplantation in the male Guinea baboon. Amer Surg 136: 206–210

    Google Scholar 

  28. González-Darder JM (1993) Experimental microsurgical repair of spinal roots. J Neurosurg 33(6): 1083–1087

    Google Scholar 

  29. Grimson BS, Ross MJ, Ryson G (1984) Return of function after intracranial suture of the trochlear nerve. Case report. J Neurosurg 61: 191–192

    PubMed  Google Scholar 

  30. I A S P (1980) Ethical standards for investigations of experimental pain in animals. Pain 9: 327–337

    Google Scholar 

  31. Jamieson AM, Eames RA (1980) Reimplantation of avulsed brachial plexus roots: an experimental study in dogs. Int J Microsur 2(2): 75–80

    Google Scholar 

  32. Kilvington (1907) An investigation on the regeneration of nerves, with regard to surgical treatment of certain paralyses. B M J 1: 988–990

    Google Scholar 

  33. Kline DG, Donner TR, Happel L, Smith B, Richter HP (1992) Intraforaminal repair of plexus spinal nerves by a posterior approach: An experimental study. J Neurosurg 76: 459–470

    PubMed  Google Scholar 

  34. Krajewski R (1992) Oculomotor nerve repair using interposed nerve graft. Neurosurgery 30: 591–594

    PubMed  Google Scholar 

  35. Liuzzi FJ, Tedeschi B (1992) Axo-glial interactions at the dorsal root transitional zone regulate neurofilament protein synthesis in axotomized sensory neurons. J Neurosci 12(12): 4783–4792

    PubMed  Google Scholar 

  36. Mesulam MM (1978) Tetramethyl benzidine for horseadish peroxidase neurohistochemistry. A non-carciogenic blue reaction-product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26: 106–117

    PubMed  Google Scholar 

  37. Millesi H (1977) Indications and results of direct surgical intervention in traction lesions of the adult brachial plexus. Rev Chir Orthop 63(1): 82–87

    PubMed  Google Scholar 

  38. Millesi H (1991) Brachial plexus injuries in adults: Operative repair. In: Gelberman RH (ed) Operative nerve repair and reconstruction, vol 1. Lippincott, Philadelphia, pp 1285–1301

    Google Scholar 

  39. Moyer EK, Kimmel DL (1943) The repair of severed motor and sensory spinal nerve roots by the arterial sleeve method of anatomosis. J Comp Neurol 88: 285–317

    Google Scholar 

  40. Moyer EK, Kimmel DL, Winborne LW (1953) Regeneration of sensory spinal nerve roots in young and senile rats. J Comp Neurol 98: 283–307

    PubMed  Google Scholar 

  41. Narakas A (1977) Indications et résultats du traitement chirurgical direct dans les lésions par elongation du plexus brachial. Rev Chir Orthop 63: 88–106

    PubMed  Google Scholar 

  42. Nathaniel EJH, Nathaniel DR (1973) Regeneration of dorsal root fibres into the adult rat spinal cord. Exp Neurol 40: 333–350

    PubMed  Google Scholar 

  43. Nathaniel EJH, Pease DC (1963) Regenerative changes in rat dorsal roots following Wallerian degeneration. J Ultrastruct Res 9: 561–567

    Google Scholar 

  44. Perkins S, Carlstedt T, Mizuno K, Aguayo AJ (1980) Failure of regenerating dorsal root axons to regrow into the spinal cord. Can J Neurol Sci 7: 323–327

    Google Scholar 

  45. Petrov MA (1971) Transplantations of nerves and roots of the spinal cord. Reconstr Surg Traumat 12: 250–262

    Google Scholar 

  46. Ramón y Cajal S (1928) Degeneration and regeneration of the nervous system, vol 1. Oxford University Press, London

    Google Scholar 

  47. Ranson SW (1914) An experimental study of Lissauer's tract and dorsal roots. J Comp Neurol 24: 531–545

    Google Scholar 

  48. Samii M (1984) Facial nerve grafting in acoustic neurinoma. Clin Plast Surg 11: 221–225

    PubMed  Google Scholar 

  49. Sanjuanbenito L, Esteban A, González-Martfnez E (1976) Regeneration of the spinal ventral roots in cats. Acta Neurochir (Wien) 34: 203–214

    Google Scholar 

  50. Sceats DJ Jr, Friedman WA, Sypert GW, Ballinger WE Jr (1986) Regeneration in peripheral nerve grafts to the cat spinal cord. Brain Res 362: 149–156

    PubMed  Google Scholar 

  51. Sedel L (1977) Palliative treatment of a series of 103 cases of paralysis from traction lesions of the brachial plexus. Rev Chir Orthop 63(7): 651–665

    PubMed  Google Scholar 

  52. Sekhar LN, Lanzino G, Sen CN, Pomins S (1992) Reconstruction of the third through sixth cranial nerves during cavernous sinus surgery. J Neurosurg 76: 935–943

    PubMed  Google Scholar 

  53. Sindou M, Gilg A, Vighetto A, Jouvet A (1992) Crytic angioma in the trochlear nerve. Excision of the invaded portion and successful repair with an autologous graft: Case report. Neurosurgery 30: 225–228

    Google Scholar 

  54. Sjøstrand J, Carlsoon CA, Thulin CA (1969) Regeneration of ventral roots. A histological study in cats. Acta Anat (Basel) 74: 532–546

    Google Scholar 

  55. Smith KJ, Kodama RT (1991) Reinnervation of denervated skeletal muscle by central neurons regenerating via ventral roots implanted into the spinal cord. Brain Res 551(1–2). 221–229

    PubMed  Google Scholar 

  56. Stensaas JL, Burgess PR, Horch KW (1979) Regenerating dorsal root axons are blocked by spinal cord astrocytes. Soc Neurosci Abstr 5: 584–670

    Google Scholar 

  57. Stephanian E, Sekhar LN, Janecka IP, Hirsch B (1992) Facial nerve repair by interposition nerve graft: Results in 22 patients. Neurosurgery 31: 73–77

    PubMed  Google Scholar 

  58. Tarlov IM (1937) Structure of the nerve root: I. Nature of the junction between the central and the peripheral nervous system. Arch Neurol Psych 37(3): 555–583

    Google Scholar 

  59. Tower SS (1931) A search for trophic influence of posterior spinal roots on skeletal muscle, with a note on the nerve fibers found in the proximal stumps of the roots after excision of the root ganglia. Brain 54: 99–110

    Google Scholar 

  60. Tower SS (1943) Regenerative capacity of ventral roots after avulsion from the spinal cord. Arch Neurol Psych 49(1): 1–12

    Google Scholar 

  61. Wynn Parry CB (1974) The management of injuries to the brachial plexus. Proc R Soc Med 67(6): 448–490

    Google Scholar 

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Authors and Affiliations

  1. Department of Anaesthesiology, Clinica Universitaria, University of Navarra, Pamplona, Navarra, Spain

    N. Sáiz-Sapena

  2. Division of Neurosurgery, Clinica Universitaria, University of Navarra, Pamplona, Navarra, Spain

    V. Vanaclocha Ph. D., M.D.

  3. Department of Anatomy, Clinica Universitaria, University of Navarra, Pamplona, Navarra, Spain

    R. Insausti

  4. Department of Pathology, Clinica Universitaria, University of Navarra, Pamplona, Navarra, Spain

    M. Idoate

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  1. N. Sáiz-Sapena
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  3. R. Insausti
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  4. M. Idoate
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Sáiz-Sapena, N., Vanaclocha, V., Insausti, R. et al. Dorsal root repair by means of an autologous nerve graft: Experimental study in the rat. Acta neurochir 139, 780–786 (1997). https://doi.org/10.1007/BF01420053

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