Summary
Peripheral nerve sheath (PNS) neoplasms, primarily neurofibromas, schwannomas and maliganant schwannomas, are among the most common tumors in fishes. Model systems involving PNS tumors in fishes are also valuable because mammalian models of PNS tumors are rare. Schwann cells, the primary cell type suspected of neoplastic transformation in these tumors, have been difficult to culture. We describe techniques for culturing normal and neoplastic Schwann cells from fish. We also present methods for preparing cells on culture dishes for electron microscopy which are especially useful when specific cells in a culture must be located for ultrastructural examination.
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Abbreviations
- PNS:
-
peripheral nerve sheath
- SC:
-
Schwann cells
References
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Schmale, M.C., Gill, K.A. & Cacal, S.M. Normal and neoplastic Schwann cells in fish. Journal of Tissue Culture Methods 16, 109–115 (1994). https://doi.org/10.1007/BF01404819
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DOI: https://doi.org/10.1007/BF01404819