Summary
The binding and uptake of fluorescence labeled wheat germ agglutinin into cytotoxic T-cells was measured by single cell cytophotometric analysis. The intensity of fluorescence in these cells increased continuously over 24 hrs, indicating a permanent turnover of the ligands for WGA. Although the labeling of the cells was intense, no change in the proliferation rate of this interleukin-2 dependent cell line was observed. Therefore no interaction between the interleukin-2 receptor and other receptors regulating the cellular proliferation with the lectin is likely.
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Abbreviations
- au:
-
arbitary units
- CTLL-1:
-
murine cytotoxic interleukin-2 dependent cell line
- FCS:
-
fetal calf serum
- FITC:
-
fluoresceinisothiocyanate
- HEPES:
-
hydroxyethylpiperazine-ethanesulfonic acid
- MHC:
-
major histocompatibility complex
- WGA:
-
wheat germ agglutinin
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Schumacher, U., Wiedemann, B. & Steinmann, G.G. Wheat germ agglutinin uptake by cytotoxic lymphocytes, a quantitative model of receptor-mediated endocytosis. Protoplasma 151, 124–127 (1989). https://doi.org/10.1007/BF01403449
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DOI: https://doi.org/10.1007/BF01403449