Abstract
A new medium consisting of UHT milk, tween 80 and agar is described for the development of both germ tube and chlamydoconidia byCandida albicans. In total 172 isolates from clinical specimens, includingC. albicans (112),C. guilliermondii (4),C. krusei (3),C. parasilopsis (16).C. tropicalis (28),Torulopsis glabrata (6) andTrichosporon beigellii (3), were examined in this medium by using the standard method. A higher percentage (98.2%) of germ tube production byC. albicans was found in this medium than in undiluted serum (90.2%). In addition, onlyC. albicans was found to be able to produce a high percentage of chlamydoconidia (95.5%) after 48 hours' incubation. In comparison with the conventional medium, corn meal tween 80 agar (21.4%), this new medium gives a significantly higher percentage and abundance of chlamydoconidia production. Being simple, cheap and easy to prepare, the new milk medium is proposed as very practical in the clinical mycology laboratory.
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References
Shepherd MG, Poulter RTM, Sullivan PA.Candida albicans: Biology, genetics and pathogenicity. Ann Rev Microbiol 1985; 39: 579–614.
Pincus DH, Salkin IF, McGinnis MR. Rapid methods in medical mycology. Lab Med 1988; 19: 315–20.
McGinnis MR. Laboratory handbook of medical mycology. New York: Academic Press, 1980.
Beheshti F, Smith AG, Krause GW. Germ tube and chlamydospore formation byCandida albicans on a new medium. J Clin Microbiol 1975; 2: 345–48.
Yong DCT, Smitka C, Prytula A, Kane J. The comparison of two agar media for germ tube and chlamydospore production byCandida albicans. Health Lab Sci 1978; 15: 197–200.
Casal M, Linares MJ. The comparison of six media for chlamydospore production byCandida albicans. Mycopathologia 1981; 76: 125–28.
Warren NG, Shadomy HJ. Yeasts of medical importance. In: Balows A, Hausler WJ Jr, Herrmann KL, Isenberg HD, Shadomy HJ, eds. Manual of clinical microbiology, 5th ed. Washington, DC: American Society for Microbiology, 1991: 17–29.
Berardinelli S, Opheim DJ. New germ tube induction medium for the identification ofCandida albicans. J Clin Microbiol 1985; 22: 861–62.
Pollack JH, Hashimoto T. Ethanol-induced germ tube formation inCandida albicans. J Gen Microbiol 1985; 131: 3303–10.
Pollock JH, Hashimoto T. The role of glucose in the pH regulation of germ tube formation inCandida albicans. Gen Microbiol 1987; 133: 415–24.
Holmes AR, Shepherd MG. Nutritional factors determine germ tube formation inCandida albicans. J Med Vet Mycol 1988; 26: 127–31.
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Jitsurong, S., Kiamsiri, S. & Pattararangrong, N. New milk medium for germ tube and chlamydoconidia production byCandida albicans . Mycopathologia 123, 95–98 (1993). https://doi.org/10.1007/BF01365086
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DOI: https://doi.org/10.1007/BF01365086