Skip to main content
SpringerLink
Log in

Genomic cloning and chromosomal assignment of rat regucalcin gene

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

The gene for a Ca2+-binding protein regucalcin was cloned from a rat genomic library which was constructed in λ FIX II by screening with radiolabeled probe (complementary DNA of rat liver regucalcin). Positive clone had 19.9 kb insert of size and contained four exons of the gene coding for a rat regucalcin. These exons included the partial coding sequence (61.2% of open reading frame) and the entire 3′-untranslated region of the gene. The nucleotide sequence of exons completely agreed with that of a rat regucalcin cDNA clone. The sequence analysis of the clone showed that the identifier sequence and two simple repeated sequences exist in the intron of the gene. Moreover, chromosomal location of the rat regucalcin gene was determined by direct R-banding fluorescencein situ hybridization (FISH) method with the 19.9 kb clone containing four exons. The regucalcin gene was localized on rat chromosome Xq11.1–12 proximal end.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Williamson JR, Cooper RK, Hoek JB: Role of calcium in the hormonal regulation of liver metabolism. Biochim Biophys Acta 639: 243–295, 1981

    Google Scholar 

  2. Reinhart PH, Taylor WM, Bygrave FL: The role of calcium ions in the mechanisms of action of α-adrenergic agonists in rat liver. Biochem J 223: 1–13, 1984

    Google Scholar 

  3. Cheung WY: Calmodulin plays a pivotal role in cellular regulation. Science 202: 19–27, 1980

    Google Scholar 

  4. Nishizuka Y: Studies and perspectives of protein kinase C. Science 233: 305–312, 1986

    Google Scholar 

  5. Yamaguchi M, Sugii K: Properties of calcium-binding protein isolated from the soluble fraction of normal rat liver. Chem Pharm Bull 29: 567–570, 1981

    Google Scholar 

  6. Yamaguchi M: A novel Ca2+-binding protein regucalcin and calcium inhibition: Regulatory role in liver cell function. In: K. Kohama (ed.). Calcium Inhibition. Japan Sci Soc Press, Tokyo and CRC Press, Boca Raton, 1992, pp 19–41

    Google Scholar 

  7. Yamaguchi M, Tai H: Inhibitory effect of calcium-binding protein regucalcin on Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase activity in rat liver cytosol. Mol Cell Biochem 106: 25–30, 1991

    Google Scholar 

  8. Yamaguchi M, Mori S: Inhibitory effect of calcium-binding protein regucalcin on protein kinase C activity in rat liver cytosol. Biochem Med Metab Biol 43: 140–146, 1990

    Google Scholar 

  9. Yamaguchi M, Sakurai T: Inhibitory effect of calcium-binding protein regucalcin on Ca2+-activated DNA fragmentation in rat liver nuclei. FEBS Lett 279: 281–284, 1991

    Google Scholar 

  10. Shimokawa N, Yamaguchi M: Molecular cloning and sequencing of the cDNA coding for a calcium-binding protein regucalcin from rat liver. FEBS Lett 327: 251–255, 1993

    Google Scholar 

  11. Shimokawa N, Isogai M, Yamaguchi M: Specific species and tissue differences for the gene expression of calcium-binding protein regucalcin. Mol Cell Biochem 143: 67–71, 1995

    Google Scholar 

  12. Shimokawa N, Yamaguchi M: Calcium administration stimulates the expression of calcium-binding protein regucalcin mRNA in rat liver. FEBS Lett 305: 151–154, 1992

    Google Scholar 

  13. Shimokawa N, Yamaguchi M: Expression of hepatic calcium-binding protein regucalcin mRNA is mediated through Ca2+/calmodulin in rat liver. FEBS Lett 316: 79–84, 1993

    Google Scholar 

  14. Yamaguchi M, Kamayama Y, Shimokawa N: Expression of calcium-binding protein regucalcin mRNA in rat liver is stimulated by calcitonin: the hormonal effect is mediated through calcium. Mol Cell Biochem 136: 43–48, 1994

    Google Scholar 

  15. Yamaguchi M, Oishi K, Isogai M: Expression of hepatic calcium-binding protein regucalcin mRNA is elevated by refeeding of fasted rats: Involvement of glucose, insulin and calcium as stimulating factors. Mol Cell Biochem 142: 35–41, 1995

    Google Scholar 

  16. Yamaguchi M, Oishi K: 17β-estradiol stimulates the expression of hepatic calcium-binding protein regucalcin mRNA in rats. Mol Cell Biochem, 143: 139–141, 1995

    Google Scholar 

  17. Isogai M, Oishi K, Shimokawa N, Yamaguchi M: Expression of hepatic calcium-binding protein regucalcin mRNA is decreased by phenolbarbital administration in rats. Mol Cell Biochem 141: 15–19, 1994

    Google Scholar 

  18. Isogai M, Shimokawa N, Yamaguchi M: Hepatic calcium-binding protein regucalcin is released into the serum of rats administered orally carbon tetrachloride. Mol Cell Biochem 131: 173–179, 1994

    Google Scholar 

  19. Isogai M, Oishi K, Yamaguchi M: Serum release of hepatic calcium-binding protein regucalcin by liver injury with galactosamine administration in rats. Mol Cell Biochem 136: 85–90, 1994

    Google Scholar 

  20. Feingerg A, Vogelstein B: A technique for labelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983

    Google Scholar 

  21. Sambrook J, Fritsch EF, Maniatis T: Analysis of genomic DNA by Southern hybridization. In: Molecular Cloning (2nd Edn), Cold Spring Habor Laboratory Press, Cold Spring Habor, New York, 1989, pp 9.31–9.53

    Google Scholar 

  22. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chainterminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977

    Google Scholar 

  23. Hattori M, Sakaki Y: Dideoxy sequencing method using denatured plasmid templates. Anal Biochem 152: 232–238, 1986

    Google Scholar 

  24. Matsuda Y, Harada Y-N, Natsuume-Saka S, Lee K, Shiomi T, Chapman VM: Location of the mouse complement factor H gene (cfh) by FISH analysis and replication R-banding. Cytogenet Cell Genet 61: 282–285, 1992

    Google Scholar 

  25. Matsuda Y, Chapman VM: Application of fluorescencein situ by bridization in genome analysis of the mouse. Electrophoresis 16: 261–272, 1995

    Google Scholar 

  26. Mount SM: A catalogue of splice junction sequences. Nucleic Acids Res 10: 459–472, 1982

    Google Scholar 

  27. Sutcliffe JG, Milner RJ, Gottesfeld JM, Lerner RA: Identifier sequences are transcribed specifically in brain. Nature 308: 237–241, 1984

    Google Scholar 

  28. Ronne M, Shibasaki Y, Poulsen BS, Anderson O: The high resolution R-banded karyotype ofRattus norvegicus. Cytogenet Cell Genet 45: 113–117, 1987

    Google Scholar 

  29. Satoh H, Yoshida MC, Sasaki M: High resolution chromosome banding in the Norway rat,Rattus norvegicus. Cytogenet Cell Genet 50: 151–154, 1989

    Google Scholar 

  30. Hamada H, Petrino MG, Kakunaga T, Seidman M, Stollar BD: Characterization of genomic poly(dT-dG) poly(dC-dA) sequences structure, organization and conformation. Mol Cell Biol 4: 2610–2621, 1984

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan

    Noriaki Shimokawa & Masayoshi Yamaguchi

  2. Division of Genetics, National Institute of Radiological Science, 4-9-1 Anagawa, Inage-ku, 263, Chiba City, Japan

    Yoichi Matsuda

Authors
  1. Noriaki Shimokawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoichi Matsuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masayoshi Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and GenBank Nucleotide Sequence Databases with the following accession number D31662

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shimokawa, N., Matsuda, Y. & Yamaguchi, M. Genomic cloning and chromosomal assignment of rat regucalcin gene. Mol Cell Biochem 151, 157–163 (1995). https://doi.org/10.1007/BF01322338

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01322338

Key Words

Search

Navigation