Abstract
The possible involvement of protein kinase C in control of ion transport was investigated on a preparation of isolated, perfused posterior gills of the Chinese crabsEriocheir sinensis (collected in 1989 from lakes near Emden, northern Germany) acclimated to fresh water. 1-oleyl-2-acetyl-sn-glycerol (OAG) and phorbol 12-myristate 13-acetate (PMA), two activators of protein kinase C, when added to the perfusion saline, induced depolarisation of the transepithelial potential difference (PD) and an increase in transepithelial Na+ influx. The observed increase was proportional to OAG concentration up to 250µM, with a 2.5× accelerated Na+ influx. OAG and PMA remained without effect on Cl− fluxes. The observed effects were in agreement with an activation, via protein kinase C, of the Na+/K+ ATPase located on the serosal side of the epithelium.
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Bianchini, A., Péqueux, A., Gilles, R. (1988). Effects of TAP and DPC on the transepithelial potential difference of isolated perfused gills of the fresh water acclimated crab,Eriocheir sinensis. Comp. Biochem. Physiol. 90A: 315–319
Bianchini, A., Gilles, R. (1990). Cyclic AMP as a modulator of NaCl transport in gills of the euryhaline Chinese crabEriocheir sinensis. Mar. Biol. 104: 191–195
Bittar, E. E., Gerard, P. R. (1987). Evidence for the presence of protein kinase C in barnacle muscle fibers. Comp. Biochem. Physiol. 88B: 687–699
Ciapa, B., Allemand, D., Payan, P. (1989). Effect of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) upon membrane ionic exchanges in sea urchin eggs. Expl Cell Res. 185: 407–418
Gilles, R., Péqueux, A., Bianchini, A. (1988). Physiological aspects of NaCl movements in the gills of the euryhaline crabEriocheir sinensis acclimated to fresh water. Comp. Biochem. Physiol. 90A: 201–207
Gocha, N., Péqueux, A., Wanson, S., Gilles, R. (1987). Cl− fluxes across isolated, perfused gills of the Chinese crabEriocheir sinensis (M. Edw.) acclimated to fresh water. Comp. Biochem. Physiol. 88A: 581–584
Greene, D. A., Lattimer, S. A. (1986). Protein kinase C agonists acutely normalize decreased ouabain-inhibitable respiration in diabetic rabbit nerve: implications for (Na, K)-ATPase regulation and diabetic complications (rapid publications). Diabetes. 35: 242–245
Hootman, S. R., Brown, M. E., Williams, J. A. (1987). Phorbol esters and A23187 regulate Na+-K+-pump activity in pancreatic acinar cells. Am. J. Physiol. 252: G499–505
Kikkawa, U., Nishizuka, Y. (1986). The role of protein kinase C in transmembrane signalling. A. Rev. Cell Biol. 2: 149–178
Lynch, C. J., Wilson, P. B., Blackmore, P. F., Eston, J. H. (1986). The hormone-sensitive hepatic Na+-pump. J. biol. Chem. 261: 14551–14556
Péqueux, A., Gilles, R. (1978). Osmoregulation of the euryhaline Chinese crabEriocheir sinensis. Ionic transports across isolated perfused gills and related to salinity of the environment. In: Me Lusky, D. S., Berry, K. J. (eds.) Physiology and behaviour of marine organisms. Pergamon Press, Oxford, p. 105–111
Péqueux, A., Gilles, R. (1981). Na+ fluxes across isolated perfused gills of the Chinese crabEriocheir sinensis. J. exp. Biol. 92: 173–186
Péqueux, A., Gilles, R., Marshall, W. S. (1988). NaCl transport in gills and related structures. Adv. comp. envirl Physiol. 1: 2–63
Yingst, D. R. (1988). Modulation of the Na,K-ATPase by Ca and intracellular proteins. A. Rev. Physiol. 50: 291–303
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Communicated by O. Kinne, Oldendorf/Luhe
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Asselbourg, V., Trausch, G., Gilles, R. et al. Protein kinase C and ion transport in the posterior gills of the Chinese crabEriocheir sinensis . Mar. Biol. 109, 115–119 (1991). https://doi.org/10.1007/BF01320237
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DOI: https://doi.org/10.1007/BF01320237