Summary
Cross hemagglutination inhibition (HI) and neuraminidase inhibition (NI) tests demonstrated strong antigenic relationship between A/Swine/Wisconsin/1/73 (SW/73) and A/Swine/Shope/15/31 (SW/31) influenza viruses. An eightyone fold purification of virus was achieved by adsorption and elution followed by differential ultracentrifugation and sedimentation through linear sucrose gradient. Radio-immunoassay using purified125I labeled viral antigens revealed antigenic variation between the two virus isolates. Neuraminidase of both viruses had pH optima between 6.5 and 7.0, and SW/31 enzyme was relatively more heat stable than SW/73.
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Contribution No. 183, Department of Infectious Diseases, and Contribution No. 1239, Division of Biology, Kansas Agricultural Experiment Station, Manhattan, Kansas 66506.
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Nath, D.M., Rodkey, L.S. & Minocha, H.C. Antigenic comparison of swine influenza virus isolates. Archives of Virology 48, 245–252 (1975). https://doi.org/10.1007/BF01317967
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DOI: https://doi.org/10.1007/BF01317967