Skip to main content
SpringerLink
Log in

Identification of potyvirus isolates from faba bean (Vicia faba L.), and the relationships between bean yellow mosaic virus and clover yellow vein virus

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Clover yellow vein virus (CYVV) isolate H and the related potyvirus isolates E178, E197, and E242 could be distinguished from bean yellow mosaic (BYMV) isolates by their wider host range among non-legume test plant species, the peculiar enlargement of the nucleolus in infected plants, and the larger size of their coat protein as evidenced by slower migration in SDS-PAGE. Serologically, they are qualitatively indistinguishable in electro-blot immunoassay (EBIA) also with antibodies specific to the N-terminal part of BYMV-B25 coat protein, implying therefore that CYVV and BYMV coat proteins contain identical amino acid sequences in the N-terminal region.

The faba-bean virus isolates from Sudan, Syria, and The Netherlands could be identified as BYMV isolates especially adapted to faba bean. All of them were weakly pathogenic toPhaseolus bean with the exception of SV205, assuming an intermediate position betweenPhaseolus-bean isolates, with low pathogenicity to faba bean, and faba-bean isolates, usually having low pathogenicity toPhaseolus bean. Strains of BYMV are thus hard to delimit.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Barnett OW, Burrows PM, McLaughlin MR, Scott SW, Baum RH (1985) Differentiation of potyviruses of the bean yellow mosaic subgroup. Acta Hortic 164: 209–216

    Google Scholar 

  2. Barnett OW, Randles JW, Burrows PM (1987) Relationships among Australian and North American isolates of the bean yellow mosaic potyvirus subgroup. Phytopathology 77: 791–799

    Google Scholar 

  3. Beczner L, Maat DZ, Bos L (1976) The relationships between pea necrosis virus and bean yellow mosaic virus. Neth J Plant Pathol 82: 41–50

    Google Scholar 

  4. Beemster ABR, van der Want JPH (1951) Serological investigations on thePhaseolus viruses 1 and 2. Antonie van Leeuwenhoek (J Microbiol Ser) 17: 15–26

    Google Scholar 

  5. Bercks R (1960) Serological relationships between beet mosaic virus, potato virus Y, and bean yellow mosaic virus. Virology 12: 311–313

    Google Scholar 

  6. Bercks R (1960) Serologische Untersuchungen zur Differenzierung von Isolaten des Phaseolus-Virus 2 und ihrer Verwandtschaft mit Phaseolus-Virus 1. Phytopathol Z 39: 120–128

    Google Scholar 

  7. Bos L (1969) Inclusion bodies of bean yellow mosaic virus, some less known closely related viruses and beet mosaic virus. Neth J Plant Pathol 75: 137–143

    Google Scholar 

  8. Bos L (1970) The identification of three new viruses isolated fromWisteria andPisum in the Netherlands, and the problem of variation within the potato virus Y group. Neth J Plant Pathol 76: 8–46

    Google Scholar 

  9. Bos L, Kowalska C, Maat DZ (1974) The identification of bean mosaic, pea yellow mosaic and pea necrosis strains of bean yellow mosaic virus. Neth J Plant Pathol 80: 173–191

    Google Scholar 

  10. Bos L, Lindsten K, Maat DZ (1977) Similarity of clover yellow vein virus and pea necrosis virus. Neth J Plant Pathol 83: 97–108

    Google Scholar 

  11. Bos L, Rubio-Huertos M (1969) Light and electron microscopy of cytoplasmic and unusual nuclear inclusion bodies evoked by a virus from necrotic peas. Virology 37: 377–385

    Google Scholar 

  12. Clark MF, Adams AM (1977) Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses. J Gen Virol 48: 311–317

    Google Scholar 

  13. Edwardson JR (1974) Some properties of the potato virus Y-group. Florida Agricult Exp Stat Monogr Ser 4. Gainesville

  14. Francki RIB (1983) Current problems in plant virus taxonomy. In: Matthews REF (ed) A critical appraisal of viral taxonomy. CRC Press, Boca Raton, Florida, pp 63–104

    Google Scholar 

  15. Geysen HM, Mason TJ, Rodda SJ (1988) Cognitive features of continuous antigenic determinants. J Mol Recogn 1: 23–41

    Google Scholar 

  16. Harrison BD (1985) Usefulness and limitations of the species concept for plant viruses. Intervirology 24: 71–78

    Google Scholar 

  17. Hollings M, Brunt AA (1981) Potyvirus group. CMI/AAB Descript Plant Vir 245

  18. Hollings M, Brunt AA (1981) Potyviruses. In: Kurstak E (ed) Handbook of plant virus infections: comparative diagnosis. Elsevier/North-Holland, Amsterdam, pp 731–807

    Google Scholar 

  19. Hollings M, Nariani TK (1965) Some properties of clover yellow vein, a virus fromTrifolium repens L. Ann Appl Biol 56: 99–109

    Google Scholar 

  20. Hollings M, Stone OM (1974) Clover yellow vein virus. CMI/AAB Descript Plant Vir 131

  21. Huttinga H (1975) Properties of viruses of the potyvirus group. 3. A comparison of buoyant density, S value, particle morphology, and molecular weight of the coat protein subunit of 10 viruses and virus isolates. Neth J Plant Pathol 81: 58–63

    Google Scholar 

  22. Jones RT, Diachun S (1977) Serologically and biologically distinct bean yellow mosaic virus strains. Phytopathology 67: 831–838

    Google Scholar 

  23. Laemmli UK, Favre M (1973) Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol 80: 575–599

    Google Scholar 

  24. Lindsten K, Brishammar S, Tomenius K (1976) Investigations on relationship and variation of some legume viruses within the potyvirus group. Meddn St Växtak Anst 16: 289–322

    Google Scholar 

  25. Lisa V, Dellavalle G (1983) Clover yellow vein virus in climbing bean (Phaseolus vulgaris L.). Phytopathol Medit 22: 49–52

    Google Scholar 

  26. Makkouk KM, Bos L, Azzam OI, Koumari S, Rizkallah A (1988) Survey of viruses affecting faba bean in six Arab countries. Arab J Plant Protect 6: 61–53

    Google Scholar 

  27. Milne RG (1988) Taxonomy of the rod-shaped filamentous viruses. In: Milne RG (ed) The filamentous plant viruses. The plant viruses 4. Plenum, New York, pp 3–50

    Google Scholar 

  28. Reddick BB, Barnett OW (1983) A comparison of three potyviruses by direct hybridization analysis. Phytopathology 73: 1506–1510

    Google Scholar 

  29. Shukla DD, Ward CW (1988) Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. J Gen Virol 69: 2703–2710

    Google Scholar 

  30. Shukla DD, Ward CW (1989) Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Adv Virus Res 36: 273–314

    Google Scholar 

  31. Shukla DD, Ward CW (1989) Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Arch Virol 106: 171–200

    Google Scholar 

  32. Shukla DD, Jilka J, Tosic M, Ford RE (1989) A novel approach to the serology of potyviruses involving affinity-purified polyclonal antibodies directed towards virus specific N-termini of coat proteins. J Gen Virol 70: 13–23

    Google Scholar 

  33. Shukla DD, Tosic M, Jilka J, Ford RE, Toler RW, Laughan MAC (1989) Taxonomy of potyviruses infecting maize, sorghum, and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology 79: 223–229

    Google Scholar 

  34. Silbernagel MJ, Jafri EM (1974) Temperature effects on curly top resistance inPhaseolus vulgaris. Phytopathology 64: 825–827

    Google Scholar 

  35. Shukla DD, Strike PH, Tracy SL, Gough KH, Ward CC (1988) The N and C termini of the coat proteins of potyviruses are surface-located and the N-terminus contains the major virus-specific epitopes. J Gen Virol 69: 1497–1508

    Google Scholar 

Download references

Author information

Author notes

  1. M. Fortass

    Present address: Department of Plant PathologyDepartment of Plant PathologyDepartment of Plant Pathology École Nationale d'Agriculture, Meknes, Morocco

Authors and Affiliations

  1. Research Institute for Plant Protection (IPO), Wageningen, The Netherlands

    M. Fortass & L. Bos

  2. Department of VirologyDepartment of VirologyDepartment of Virology Agricultural University, Wageningen, The Netherlands

    R. W. Goldbach

Authors
  1. M. Fortass
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Bos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. W. Goldbach
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fortass, M., Bos, L. & Goldbach, R.W. Identification of potyvirus isolates from faba bean (Vicia faba L.), and the relationships between bean yellow mosaic virus and clover yellow vein virus. Archives of Virology 118, 87–100 (1991). https://doi.org/10.1007/BF01311305

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01311305

Keywords

Search

Navigation