Summary
Feline leukemia virus (FeLV) is a horizontally transmitted agent of the domestic cat which is known to be associated with wide spectrum of diseases of the hematopoietic system. In the present study, proviral DNAs of FeLV proviruses were examined in the tumor cells of natural killer cell lineage which is very rare in cats. In the chromosomal DNA of the tumor cells, 5 distinct bands corresponding to exogenous FeLV provirus genomes were detected by digestion withEcoRI which does not cut most FeLV isolates. Five clones of pLC1, pLC2, pLC3, pLC4, and pLC5 obtained from the 5 respective bands were analysed by restriction endonuclease mapping and Southern blot hybridization using gene-specific probes of FeLV. The results have clearly demonstrated that pLC4 and pLC5 contained large deletions in thepol and part ofgag regions, while the full-length proviruses could be observed in pLC1 and pLC2. Furthermore, pLC3 contained part of a variant FeLV genome having anEcoRI site in itsgag region. The molecular clones of defective and variant FeLV in this study may be useful for the further examination of tumorigenesis of large granular lymphoma in the cat.
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References
Aziz DC, Hanna Z, Jolicoeur P (1989) Severe immunodeficiency disease induced by a defective murine leukaemia virus. Nature 338: 505–508
Casey JW, Roach A, Mullins JI, Burck KB, Nicolson MO, Gardner MB, Davidson N (1981) The U3 portion of feline leukemia virus DNA identifies horizontally acquired proviruses in leukemic cats. Proc Natl Acad Sci USA 78: 7778–7782
Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81: 1991–1995
Elder JH, Mullins JI (1983) Nucleotide sequence of the envelope gene of Gardner-Arnstein feline leukemia virus B reveals unique sequence homologies with a murine mink cell focus-forming virus. J Virol 46: 871–880
Fulton R, Forrest D, McFarlane R, Onions D, Neil JC (1987) Retroviral transduction of T-cell antigen receptor beta-chain andmyc genes. Nature 326: 190–194
Goitsuka R, Tsuji M, Matsumoto M, Onda C, Matsuoka K, Yokomori K, Yasuda K, Hirota Y, Ono K, Hayashi T, Hasegawa A (1988) A case of feline large granular lymphoma. Jpn J Vet Sci 50: 593–595
Hardy WD Jr, Old LJ, Hess PW, Essex M, Cotter S (1973) Horizontal transmission of feline leukemia virus. Nature 244: 266–269
Hoover EA, Kociba GJ, Hardy WD Jr, Yohn DS (1974) Erythroid hypoplasia in cats inoculated with feline leukemia virus. J Natl Cancer Inst 53: 1271–1276
Hoover EA, Mullins JI, Quackenbush SL, Gasper PW (1987) Experimental transmission and pathogenesis of immunodeficiency syndrome in cats. Blood 70: 1880–1892
Jarret O, Hardy WD Jr, Golder MC, Hay D (1978) The frequency of occurrence of feline leukaemia virus subgroups in cats. Int J Cancer 21: 334–337
Jarrett W, Jarrett O, Mackey L, Laird H, Hardy W Jr, Essex M (1973) Horizontal transmission of leukemia virus and leukemia in the cat. J Natl Cancer Inst 51: 833–841
Kobayashi N, Konishi H, Sabe H, Shigesada K, Noma T, Honjo T, Hatanaka M (1984) Genomic structure of HTLV (human T-cell leukemia virus): detection of defective genome and its amplification in MT-2 cells. EMBO J 3: 1339–1343
Levy LS, Gardner MB, Casey JW (1984) Isolation of a feline leukaemia provirus containing the oncogenemyc from a feline lymphosarcoma. Nature 308: 853–856
Mackey L, Jarrett W, Jarrett O, Laird H (1975) Anemia associated with feline leukemia virus infection in cats. J Natl Cancer Inst 54: 209–217
Miura T, Shibuya M, Tsujimoto H, Fukasawa M, Hayami M (1989) Molecular cloning of a feline leukemia provirus integrated adjacent to the c-myc gene in a feline T-cell leukemia cell line and the unique structure of its long terminal repeat. Virology 169: 458–461
Miura T, Tsujimoto H, Fukasawa M, Kodama T, Shibuya M, Hasegawa A, Hayami M (1987) Structural abnormality and overexpression of themyc gene in feline leukemias. Int J Cancer 40: 564–569
Mullins JI, Brody DS, Binari RC Jr, Cotter SM (1984) Viral transduction of c-myc gene in naturally occurring feline leukemias. Nature 308: 856–858
Mullins JI, Casey JW, Nicolson MO, Burck KB, Davidson N (1981) Sequence arrangement and biological activity of cloned feline leukemia virus proviruses from a virus-productive human cell line. J Virol 38: 688–703
Mullins JI, Chen CS, Hoover EA (1986) Disease-specific and tissue-specific production of unintegrated feline leukemia virus variant DNA in feline AIDS. Nature 319: 333–336
Neil JC, Hughes D, McFarlane R, Wilkie NM, Onions DE, Lees G, Jarrett O (1984) Transduction and rearrangement of themyc gene by feline leukemia virus in naturally occurring T-cell leukemias. Nature 308: 814–820
Onions D, Jarrett O, Testa N, Frassoni F, Toth S (1982) Selective effect of feline leukemia virus on early erythroid precursors. Nature 296: 156–158
Overbaugh J, Donahue PR, Quackenbush SL, Hoover EA, Mullins JI (1988) Molecular cloning of a feline leukemia virus that induces fatal immunodeficiency disease in cats. Science 239: 906–910
Rickard CG, Post JE, Noronha F, Barr LM (1969) A transmissible virus-induced lymphocytic leukemia of the cat. J Natl Cancer Inst 42: 987–1014
Riedel N, Hoover EA, Dornsife RE, Mullins JI (1988) Pathogenic and host range determinants of the feline aplastic anemia retrovirus. Proc Natl Acad Sci USA 85: 2758–2762
Riedel N, Hoover EA, Gasper PW, Nicolson MO, Mullins JI (1986) Molecular analysis and pathogenesis of the feline aplastic anemia retrovirus, feline leukemia virus C-Sarma. J Virol 60: 242–250
Sarma PS, Log T (1973) Subgroup classification of feline leukemia viruses by viral interference and neutralization tests. Virology 54: 160–169
Sherr CJ, Fedele LA, Oskarsson M, Maizel J, Wonde GV (1980) Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus. J Virol 34: 200–212
Southern EM (1975) Detection of specific sequences among DNA fragments by gel electrophoresis. J Mol Biol 98: 503–517
Stewart MA, Warnock M, Wheeler A, Wilkie N, Mullins JI, Onions DE, Neil JC (1986) Nucleotide sequence of a feline leukemia virus subgroup A envelope gene and long terminal repeat and evidence for the recombinational origin of subgroup B viruses. J Virol 58: 825–834
Vieira J, Messing J (1987) Production of single-stranded plasmid DNA. In: Wu R, Grossman L (eds) Methods in enzymology, vol 153. Academic Press, New York, pp 3–11
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Matsumoto, Y., Tsujimoto, H., Fukasawa, M. et al. Molecular cloning of feline leukemia provirus genomes integrated in the feline large granular lymphoma cells. Archives of Virology 111, 177–185 (1990). https://doi.org/10.1007/BF01311052
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DOI: https://doi.org/10.1007/BF01311052