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Evidence for retrovirus in miniature swine with radiation-induced leukemia or metaplasia

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Summary

Biochemical and morphological evidence indicates that a type-C retrovirus is present in the blood of swine (both leukemic and nonleukemic) exposed to strontium-90 radiation. Nonexposed swine that were leukemic also had virus present. The virus was shown to contain an RNase-sensitive DNA polymerase activity with cation, detergent and template requirements similar to those of known viral reverse transcriptases. The buoyant density of the virus was 1.14 to 1.16 g/ml, which can be converted, by treatment with ether, to a virion core having a density of 1.20 to 1.23 g/ml.

Linear regression analysis indicated a correlation between virus-associated DNA polymerase activity and the number of blast cells in the peripheral blood.

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Authors and Affiliations

  1. Biology and Chemistry Department, Pacific Northwest Laboratory, Richland, Washington, U.S.A.

    M. E. Frazier

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With 5 Figures

Work supported by the U.S. Department of Energy under Contract DE-ACO 6-76 RLO-1830.

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Frazier, M.E. Evidence for retrovirus in miniature swine with radiation-induced leukemia or metaplasia. Archives of Virology 83, 83–97 (1985). https://doi.org/10.1007/BF01310966

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  • DOI: https://doi.org/10.1007/BF01310966

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