Summary
A new method for permeabilizing the plasmalemma of theNitella internodal cell was developed. When electrical pulses were applied to the cell in a medium lacking Ca2+, the plasmalemma was quickly permeabilized and became freely permeable to ATP. The advantage of the present method over previous methods (Shimmen andTazawa 1982b, 1983) is discussed.
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Abbreviations
- ATP:
-
adenosine 5′-triphosphoric acid
- EGTA:
-
ethyleneglycol-bis-(β-aminoethyl ether)N, N′-tetraacetic acid
- HMM:
-
heavy meromyosin
- PIPES:
-
piperazine-N,N′-bis(2-ethanesulfonic acid)
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Shimmen, T., Tazawa, M. Permeabilization ofNitella internodal cell with electrical pulses. Protoplasma 117, 93–96 (1983). https://doi.org/10.1007/BF01281789
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DOI: https://doi.org/10.1007/BF01281789