Summary
A new method for permeabilizing the plasmalemma of theNitella internodal cell was developed. When electrical pulses were applied to the cell in a medium lacking Ca2+, the plasmalemma was quickly permeabilized and became freely permeable to ATP. The advantage of the present method over previous methods (Shimmen andTazawa 1982b, 1983) is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ATP:
-
adenosine 5′-triphosphoric acid
- EGTA:
-
ethyleneglycol-bis-(β-aminoethyl ether)N, N′-tetraacetic acid
- HMM:
-
heavy meromyosin
- PIPES:
-
piperazine-N,N′-bis(2-ethanesulfonic acid)
References
Adams, R. J., 1982: Organella movement in axons depends on ATP. Nature297, 327–329.
Baker, P. F., Knight, D. E., 1978: Calcium-dependent exocytosis in bovine adrenal medullary cells with leaky plasma membranes. Nature276, 620–622.
Hayama, T., Tazawa, M., 1978: Cessation of cytoplasmic streaming accompanying osmosis-induced membrane depolarization inNitella flexilis. Cell Structure and Function3, 47–60.
Hill, S. E., Osterhout, W. J., 1938: Delayed potassium effect inNitella. J. gen. Physiol.22, 107–113.
Kamiya, N., 1981: Physical and chemical basis of cytoplasmic streaming. Ann. Rev. Plant Physiol.32, 205–236.
—,Kuroda, K., 1956: Velocity distribution of the protoplasmic streaming inNitella cells. Bot. Mag. Tokyo69, 543–554.
Kinoshita, K., Jr., Tsong, T. Y., 1978: Survival of sucrose-loaded erythrocytes in the circulation. Nature272, 258–260.
Nothnagel, E. A., Barak, L. S., Sanger, J. S., Webb, W. W., 1981: Fluorescence studies on modes of cytochalasin B and phallotoxin action on cytoplasmic streaming inChara. J. Cell Biol.88, 364–372.
Riemann, F., Zimmermann, U., Pilwat, G., 1975: Release and uptake of haemoglobin and ions in red blood cells induced by dielectric breakdown. Biochim. biophys. Acta394, 449–462.
Shimmen, T., 1978: Dependency of cytoplasmic streaming on intracellular ATP and Mg2 concentrations. Cell Structure and Function3, 113–121.
—,Tazawa, M., 1982 a: Effects of intracellular vanadate on electrogenesis, excitability and cytoplasmic streaming inNitellopsis obtusa. Plant Cell Physiol.23, 669–677.
— —, 1982 b: Cytoplasmic streaming in the cell model ofNitella. Protoplasma112, 101–106.
— —, 1983: Control of cytoplasmic streaming by ATP, Mg2+ and cytochalasin B in permeabilizedCharaceae cell. Protoplasma115, 18–24.
Tazawa, M., Kikuyama, M., Shimmen, T., 1976: Electric characteristics and cytoplasmic streaming ofCharaceae cells lacking tonoplast. Cell Structure and Function1, 165–176.
Tazawa, M., Kishimoto, U., 1968: Cessation of cytoplasmic streaming ofChara internode during action potential. Plant Cell Physiol.9, 361–368.
—,Shimmen, T., 1982: Artificial control of cytoplasmic pH and its bearing on cytoplasmic streaming, electrogenesis and excitability ofCharaceae cells. Bot. Mag. Tokyo95, 147–154.
Tominaga, Y., Shimmen, T., Tazawa, M., 1983: Control of cytoplasmic streaming by extracellular Ca2+ in permeabilizedNitella cells. Protoplasma116, 75–77.
—,Tazawa, M., 1981: Reversible inhibition of cytoplasmic streaming by intracellular Ca2+ in tonoplast-free cells ofChara australis. Protoplasma109, 103–111.
Williamson, R. E., 1975: Cytoplasmic streaming inChara: A cell model activated by ATP and inhibited by cytochalasin B. J. Cell Sci.17, 655–668.
—,Toh, B. H., 1979: Motile models of plant cells and the immunofluorescent localization of actin in a motileChara cell model. In: Cell Motility: Molecules and Organization (Hatano, S., Ishikawa, H., Sato, H., eds.), pp. 339–346. Tokyo: University of Tokyo Press.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shimmen, T., Tazawa, M. Permeabilization ofNitella internodal cell with electrical pulses. Protoplasma 117, 93–96 (1983). https://doi.org/10.1007/BF01281789
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01281789