Skip to main content
SpringerLink
Log in

The 35S-CaMV promoter is silent during early embryogenesis but activated during nonembryogenic sporophytic development in microspore culture

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

The cauliflower mosaic virus 35S (35S-CaMV) promoter, which is generally used as a constitutive promoter in plants, is known to be silent during microspore and pollen development. Here we analyzed whether the 35S-CaMV promoter fused to thegus (β-glucuronidase) gene can be used as a marker for early sporophytic development in embryogenic microspore cultures of tobacco andBrassica napus. In microspore culture ofB. napus, the 35S-CaMV promoter remained off from the start of embryogenic culture up to the mid-cotyledonary embryo stage. 35S-CaMV promoter activity was only present in those microspores that initiated sporophytic development, but failed to enter embryogenic development. Similar results were also obtained with shed-microspore cultures of tobacco, in which rapid, direct embryogenesis takes place. In isolated-microspore cultures, in which embryogenesis is delayed, an intermitting period of sporophytic development was observed, characterized by extensive 35S-CaMV promoter activity. Therefore, the 35S-CaMV promoter discriminates between two classes of sporophytic development: it is activated in microspores which change fate from gametophytic into (temporarily) nonembryogenic sporophytic development, whereas the promoter is silent in sporophytic microspores that enter embryogenic development directly. This mirrors our observation that the 35S-CaMV promoter is also silent in young zygotic embryos.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Benfey PN, Chua NH (1989) The cauliflower mosaic virus 35S-CaMV promoter: combinatorial regulation of transcription in plants. Science 250: 959–966

    Google Scholar 

  • Cordewener JHG, Custers JBM, Dons HJM, van Lockeren Campagne MM (1996) Molecular and biochemical events during the induction of microspore embryogenesis. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 1. Kluwer, Dordrecht, pp 111–124

    Google Scholar 

  • Custers JBM, Cordewener JHG, Nöllen Y, Dons HJM, van Lookeren Campagne MM (1994) Temperature controls both gametophytic and sporophytic development in microspore cultures ofBrassica napus. Plant Cell Rep 13: 267–271

    Google Scholar 

  • — —, Dons HJM, van Lookeren Campagne MM (1996) Regulation of the inductive phase of microspore embryogenesis inBrassica napus. Acta Hort 407: 209–217

    Google Scholar 

  • —, Oldenhof MT, Schrauwen JAM, Cordewener JHG, Wullems GJ, van Lookeren Campagne MM (1997) Analysis of microspore-specific promoters in transgenic tobacco. Plant Mol Biol 35: 689–699

    PubMed  Google Scholar 

  • De Block M, de Brouwer D, Tenning P (1989) Transformation ofBrassica napus andBrassica oleracea usingAgrobacterium tumefaciens and the expression of thebar andneo genes in transgenic plants. Plant Physiol 91: 694–701

    Google Scholar 

  • Ellis DD, McCabe D, Russell D, Martinell B, McCown BH (1991) Expression of inducible angiosperm promoters in a gymnosperm,Picea glauca (white spruce). Plant Mol Biol 17: 19–27

    PubMed  Google Scholar 

  • Ferrie AMR, Palmer CE, Keller WA (1994) Biotechnological applications of haploids. In: Shargool PD, Ngo TT (eds) Biotechnological applications of plant cultures. CRC Press, Boca Raton, pp 77–110

    Google Scholar 

  • Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231

    Google Scholar 

  • Hunold R, Burrus M, Bronner R, Duret JP, Hahne G (1995) Transient gene expression in sunflower (Helianthusannuus L.) following microprojectile bombardement. Plant Sci 105: 95–109

    Google Scholar 

  • Khush GS, Virmani SS (1996) Haploids in plant breeding. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 1. Kluwer, Dordrecht, pp 11–33

    Google Scholar 

  • Kyo M, Harada H (1986) Control of the developmental pathway of tobacco pollen in vitro. Planta 168: 427–432

    Google Scholar 

  • Loeb TA, Reynolds TL (1994) Transient expression of theuidA gene in pollen embryoids of wheat following microprojectile bombardement. Plant Sci 104: 81–91

    Google Scholar 

  • Mascarenhas JP, Hamilton DA (1992) Artifacts in the localization of GUS activity in anthers of petunia transformed with a CaMV 35S-GUS construct. Plant J 3: 405–408

    Google Scholar 

  • Moore PJ, Moore AJ, Collins GB (1994) Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons. Plant Cell Rep 13: 556–560

    Google Scholar 

  • Morrison RA, Evans DA, Fan Z (1991) Haploid plants from tissue culture: application in crop improvement. In: Biswas BB, Harris JR (eds) Subcellular biochemistry, vol 17, plant genetic engineering. Plenum, New York, pp 53–72

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–479

    Google Scholar 

  • Odell JT, Hoopes JL, Vermerris W (1994) Seed-specific gene activation mediated by the Cre/lox site-specific recombination system. Plant Physiol 106: 447–458

    PubMed  Google Scholar 

  • Reinert J, Bajaj YPS, Heberle E (1975) Induction of haploid tobacco plants from isolated pollen. Protoplasma 84: 191–196

    Google Scholar 

  • Shepherd RJ (1981) Cauliflower mosaic virus. Holywell Press, Oxford (CMI/AAB description of plant viruses, no 243)

    Google Scholar 

  • Stöger E, Benito Moreno RM, Ylstra B, Vicente O, Heberle-Bors E (1992) Comparison of different techniques for gene transfer into mature and immature tobacco pollen. Transgenic Res 1: 71–78

    Google Scholar 

  • Töpfer R, Matzeit V, Gronenborn B, Schell J, Steinbiss HH (1987) A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Res 15: 5990

    Google Scholar 

  • Touraev A, Ilham A, Vicente O, Heberle-Bors E (1996) Stress-induced microspore embryogenesis in tobacco: an optimized system for molecular studies. Plant Cell Rep 15: 561–565

    Google Scholar 

  • —, Vicente O, Heberle-Bors E (1997) Initiation of microspore embryogenesis by stress. Trends Plant Sci 2: 297–302

    Google Scholar 

  • Twell D, Klein TM, Fromm ME, McCormick (1989) Transient expression of chimeric genes delivered into pollen by microprojectile bombardement. Plant Physiol 91: 1270–1274

    Google Scholar 

  • Uwer U, Wilmitzer L, Altmann T (1998) Inactivation of a glycyl-tRNA synthetase leads to an arrest in plant embryo development. Plant Cell 10: 1277–1294

    PubMed  Google Scholar 

  • Vancanneyt G, Schmidt R, O'Conner-Sanchez A, Willmitzer L, Rocha-Sosa M (1990) Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events inAgrobacterium-mediated plant transformation. Mol Gen Genet 220: 245–250

    PubMed  Google Scholar 

  • Wilkinson JE, Twell D, Lindsey K (1997) Activities of CaMV 35S and nos promoters in pollen: implications for field release of transgenic plants. J Exp Bot 48: 265–275

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centrum voor Plantenveredelingsen Reproduktieonderzoek, Dienst Landbouwkundig Onderzoek, Postbus 16, 6700, AA Wageningen, The Netherlands

    J. B. M. Custers, S. C. H. J. Snepvangers, H. J. Jansen, L. Zhang & M. M. van Lookeren Campagne

Authors
  1. J. B. M. Custers
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. C. H. J. Snepvangers
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. J. Jansen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. M. van Lookeren Campagne
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. B. M. Custers.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Custers, J.B.M., Snepvangers, S.C.H.J., Jansen, H.J. et al. The 35S-CaMV promoter is silent during early embryogenesis but activated during nonembryogenic sporophytic development in microspore culture. Protoplasma 208, 257–264 (1999). https://doi.org/10.1007/BF01279097

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01279097

Keywords

Search

Navigation