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Characterization of the effects of serotonin on the release of [3H]dopamine from rat nucleus accumbens and striatal slices

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Abstract

The effect of serotonin agonists on the depolarization (K+)-induced, calcium-dependent, release of [3H]dopamine (DA) from rat nucleus accumbens and striatal slices was investigated. Serotonin enhanced basal3H overflow and reduced K+-induced release of [3H]DA from nucleus accumbens slices. The effect of serotonin on basal3H overflow was not altered by the serotonin antagonist, methysergide, or the serotonin re-uptake blocker, chlorimipramine, but was reversed by the DA re-uptake carrier inhibitors nomifensine and benztropine. With the effect on basal overflow blocked, serotonin did not modulate K+-induced release of [3H]DA in the nucleus accumbens or striatum. The serotonin agonists, quipazine (in the presence of nomifensine) and 5-methoxytryptamine, did not significantly affect K+-induced release of [3H]DA in the nucleus accumbens. This study does not support suggestions that serotonin receptors inhibit the depolarization-induced release of dopamine in the nucleus accumbens or striatum of the rat brain. The present results do not preclude the possibility that serotonin may affect the mesolimbic reward system at a site which is post-synaptic to dopaminergic terminals in the nucleus accumbens.

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References

  1. Fibiger, H. C., and Phillips, A. G. 1981. Increased intracranial self-stimulation in rats after long-term administration of desipramine. Science 214:683–685.

    Google Scholar 

  2. Willner, P. 1983. Dopamine and depression: A review of recent evidence. II. Theoretical approaches. Brain Res. Rev. 6:225–236.

    Google Scholar 

  3. Nurse, B., Russell, V. A., and Taljaard, J. J. F. 1984. 406-1 and β-Adrenoceptor agonists modulate [3H] dopamine release from rat nucleus accumbens slices: Implications for research into depression. Neurochem. Res. 9:1231–1238.

    Google Scholar 

  4. Nurse, B., Russell, V. A., and Taljaard, J. J. F. 1986. Effect of chronic desipramine treatment on adrenoceptor modulation of [3H] dopamine release from rat nucleus accumbens slices. Brain Res. 334:235–242.

    Google Scholar 

  5. Russell, V. A., Nurse, B., Lamm, M. C. L., and Taljaard, J. J. F. 1987. Effect of chronic antidepressant treatment on noradrenergic modulation of [3H] dopamine release from rat nucleus accumbens and striatal slices. Brain Res. 410:78–82.

    Google Scholar 

  6. Azmitia, E. C., and Segal, M. 1978. An autoradiographic analysis of the differential ascending projections of the dorsal and median raphe nuclei in the rat. J. Comp. Neurol. 179:641–668.

    Google Scholar 

  7. Lorens, S. A., and Guldberg, H. C. 1974. Regional 5-hydroxytryptamine following selective midbrain raphe lesions in the rat. Brain Res. 78:45–56.

    Google Scholar 

  8. Vandermaelen, C. P., Bonduki, A. C., and Kitai, S. T. 1979. Excitation of caudate-putamen neurons following stimulation of the dorsal raphe nucleus in the rat. Brain Res. 175:356–361.

    Google Scholar 

  9. De Belleroche, J., and Gardiner, I. M. 1982. Contrasting effects of 5-hydroxytryptamine on the release of dopamine and acetylcholine in the nucleus accumbens of rat. J. Neural. Transm. 55:227–242.

    Google Scholar 

  10. De Belleroche, J. S., and Bradford, H. F. 1980. Presynaptic control of the synthesis and release of dopamine from striatal synaptosomes: A comparison between the effects of 5-hydroxytryptamine, acetylcholine and glutamate. J. Neurochem. 35:1227–1234.

    Google Scholar 

  11. Ennis, C., Kemp, J. D., and Cox, B. 1981. Characterization of inhibitory 5-hydroxytryptamine receptors that modulate dopamine release in the striatum. J. Neurochem. 36:1515–1520.

    Google Scholar 

  12. Westfall, T. C., and Tittermary, V. 1982. Inhibition of the electrically induced release of [3H] dopamine by serotonin from superfused rat striatal slices. Neurosci. Lett. 28:205–209.

    Google Scholar 

  13. Jones, D. L., Mogenson, G. T., and Wu, M. 1981. Injections of dopaminergic, cholinergic, serotonergic and GABAergic drugs into the nucleus accumbens: effects on locomotor activity in the rat. Neuropharmacology 20:29–37.

    Google Scholar 

  14. Giambalvo, C. T., and Snodgrass, S. R. 1978. Biochemical and behavioural effects of serotonin neurotoxins on the nigrostriatal dopamine system: comparison of injection sites. Brain Res. 152:555–556.

    Google Scholar 

  15. James, T. A., and Starr, M. S. 1980. Rotational behaviour elicited by 5-HT in the rat: evidence for an inhibitory role of 5-HT in the substantia nigra and corpus striatum. J. Pharm. Pharmacol. 32:196–200.

    Google Scholar 

  16. Spampinato, U., Invernizzi, R., and Samanin, R. 1984. Evidence of serotonin involvement in the effect of morphine on dopamine metabolism in the rat nucleus accumbens but not in the striatum. Pharmacol. Res. Commun. 16:519–523.

    Google Scholar 

  17. Alpert, B. A., and Iversen, S. D. 1983. Striatal and limbic dopamine function: evidence from animal studies. Ann. Neurol. 13:578.

    Google Scholar 

  18. Missale, C., Castelletti, L., Govoni, S., Spano, P. F., Trabucchi, M., and Hanbauer, I. 1985. Dopamine uptake is differentially regulated in rat striatum and nucleus accumbens. J. Neurochem. 45:51–56.

    Google Scholar 

  19. Hoffmann, I. S., Naylor, R. J., and Cubeddu, L. X. 1980. Presynaptic effects of 2-aminotetralins on striatal dopaminergic neurons. J. Pharmacol. Exp. Therap. 215:486–493.

    Google Scholar 

  20. Richelson, E., and Pfenning, M. 1984. Blockade by antidepressants and related compounds of biogenic amine uptake into rat brain synaptosomes: most antidepressants selectively block norepinephrine uptake. Eur. J. Pharmacol. 104:277–286.

    Google Scholar 

  21. Dubocovich, M. L., and Zahniser, N. R. 1985. Binding characteristics of the dopamine uptake inhibitor [3H] nomifensine to striatal membranes. Biochem. Pharmacol. 34:1137–1144.

    Google Scholar 

  22. Liang, N. Y., and Rutledge, C. O. 1982. Evidence for carrier-mediated efflux of dopamine from corpus striatum. Biochem. Pharmacol. 31:2479–2484.

    Google Scholar 

  23. Raiteri, M., Bonanno, G., Marchi, M., and Maura, G. 1984. Is there a functional linkage between neurotransmitter uptake mechanisms and presynaptic receptors? J. Pharmacol. Exp. Therap. 231:671–677.

    Google Scholar 

  24. Raiteri, M., Cerrito, F., Cervoni, A. M., and Levi, G. 1979. Dopamine can be released by two mechanisms differentially affected by the dopamine transport inhibitor nomifensine. J. Pharmacol. Exp. Therap. 208:195–202.

    Google Scholar 

  25. Niddam, R., Arbilla, S., Scatton, B., Dennis, T., and Langer, S. Z. 1985. Amphetamine induced release of endogenous dopamine in vitro is not reduced following pretreatment with reserpine. Naunyn-Schmiedeberg's Arch. Pharmacol. 329:123–127.

    Google Scholar 

  26. Peroutka, S. J., and Snyder, S. H. 1983. Multiple serotonin receptors and their physiological significance. Fed. Proc. 42:213–217.

    Google Scholar 

  27. Shaskan, E. G., and Snyder, S. H. 1970. Kinetics of serotonin accumulation into slices from rat brain: relationship to catecholamine uptake. J. Pharmacol. Exp. Therap. 175:404–418.

    Google Scholar 

  28. Feuerstein, T. J., Hertting, G., Lupp, A., and Neufang. 1986. False labelling of dopaminergic terminals in the rabbit caudate nucleus: uptake and release of [3H]-5-hydroxytryptamine. Br. J. Pharmacol. 88:677–684.

    Google Scholar 

  29. Andrews, D. W., Patrick, R. L., and Barchas, J. D. 1978. The effects of 5-hydroxytryptophan and 5-hydroxytryptamine on dopamine synthesis and release in rat brain striatal synaptosomes. J. Neurochem. 30:465–470.

    Google Scholar 

  30. Zumstein, A. Z., Karduck, W., and Starke, K. 1981. Pathways of dopamine metabolism in the rabbit caudate nucleus in vitro. Naunyn-Schmiedeberg's Arch. Pharmacol. 316:205–217.

    Google Scholar 

  31. Spampinato, U., Esposito, E., and Samanin, R. 1985. Serotonin agonists reduce dopamine synthesis in the striatum only when the impulse flow of nigrostriatal neurons is intact. J. Neurochem. 45:980–982.

    Google Scholar 

  32. Blier, P., and De Montigny, C. 1983. Effects of quipazine on pre- and postsynaptic serotonin receptors: single cell studies in the rat CNS. Neuropharmacology 22:495–499.

    Google Scholar 

  33. Medon, P. J., Leeling, J. L., and Phillips, B. M. 1973. Influence of quipazine, a potential anti-parkinsonian agent on the uptake of3H-dopamine and3H-serotonin into rat striatal tissue in vitro. Life Sci. 13:685–691.

    Google Scholar 

  34. Hetey, L., Schwitzkowsky, R., and Oelssner, W. 1983. Influence of psychotomimetics and lisuride on synaptosomal dopamine release in the nucleus accumbens of rats. Eur. J. Pharmacol. 93:213–220.

    Google Scholar 

  35. Wise, R. A. 1978. Neuroleptic attenuation of intracranial self-stimulation: reward or performance deficits? Life Sci. 22:535–542.

    Google Scholar 

  36. Strecker, R. E., Roberts, D. C. S., and Koob, G. F. 1982. Apomorphine-induced facilitation of intracranial self-stimulation following dopamine denervation of the nucleus accumbens. Pharmacol. Biochem. Behav. 17:1015–1018.

    Google Scholar 

  37. Prado-Alcala, R., Streather, A., and Wise, R. A. 1984. Brain stimulation reward and dopamine terminal fields. II. Septal and cortical projections. Brain Res. 301:209–219.

    Google Scholar 

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Authors and Affiliations

  1. MRC Research Unit for the Neurochemisty of Mental Diseases, Department of Chemical Pathology, University of Stellenbosch, P.O. Box 63, 7505, Tygerberg, Republic of South Africa

    B. Nurse, V. A. Russell & J. J. F. Taljaard

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  2. V. A. Russell
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  3. J. J. F. Taljaard
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Nurse, B., Russell, V.A. & Taljaard, J.J.F. Characterization of the effects of serotonin on the release of [3H]dopamine from rat nucleus accumbens and striatal slices. Neurochem Res 13, 403–407 (1988). https://doi.org/10.1007/BF01268873

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