Summary
Venezuelan equine encephalomyelitis (VEE) virus grown in chick embryo fibroblasts was concentrated by differential centrifugation and purified in sucrose and caesium chloride density gradients. Treatment of the virus with Tween 80 and ether appeared to be the optimal method for obtaining biologically active subviral components. In experiments with sucrose density gradient centrifugation the sedimentation coefficient of the virus was about 380 S and that of virus ribonucleoprotein (RNP) was about 160 S. In experiments with caesium chloride equilibrium density centrifugation virions banded at the buoyant density of 1.25 g/cm3 and virus RNP banded at 1.42 g/cm3.
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References
Aaslestad, H. G., E. J. Hoffman, andA. Brown: Fractionation of Eastern equine encephalitis virus by density gradient centrifugation in CsCl. J. Virol.2, 972 (1968).
Clarke, D. H., andJ. Casals: Techniques for hemagglutination and hemagglutination inhibition with arthropod-borne virus. Amer. J. trop. Med. Hyg.7, 561 (1958).
Dobos, P., andP. Faulkner: Characterization of a cytoplasmic fraction from Sindbis virus-infected cultures. Canad. J. Microbiol.15, 215 (1969).
Gröschel, D., andE. Wecker: Further characterization of a subviral carrier of viral RNA in Western Equine encephalitis infected tissue cultures. Arch. ges. Virusforsch.17, 669 (1965).
Faulkner, P., andP. Dobos: Investigations on the formation and interconversion of Sindbis virus hemagglutinins. Canad. J. Microbiol.14, 45 (1968).
Faulkner, P., andS. M. McGee-Russel: Purification and structure of Semliki Forest virus isolated from mouse brain. Canad. J. Microbiol.14, 153 (1968).
Friedman, R. M., andJ. K. Berezesky: Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication. J. Virol.1, 374 (1967).
Klimenko, S. M., F. I. Yeeshov, Y. P. Gofman, A. P. Nabatnikov, andV. M. Zhdanov: Architecture of Venezuelan Equine Encephalomyelitis Virus. Virology27, 125 (1965).
Martin, R. G., andB. N. Ames: A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J. biol. Chem.236, 1372 (1961).
Mussgay, M., andJ. Weibel: Electron microscopic demonstration of purified Venezuelan equine encephalitis virus. Virology19, 109 (1963).
Porterfield, J. S.: Plaque production with yellow fever and related arthropodborne viruses. Nature (Lond.)183, 1069 (1959).
Zhdanov, V. M., F. I. Yershov, andL. V. Uryvatev: Ribonucleic acids synthesized in Venezuelan equine encephalomyelitis virus-infected cells. Virology38, 355 (1969).
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Uryvayev, L.V., Zhdanov, V.M., Yershov, F.I. et al. Characteristics of venezuelan equine encephalomyelitis virus ribonucleoprotein. Archiv f Virusforschung 33, 281–287 (1971). https://doi.org/10.1007/BF01254684
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DOI: https://doi.org/10.1007/BF01254684