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Identification of RFLP markers closely linked to the bolting gene B and their significance for the study of the annual habit in beets (Beta vulgaris L.)

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Abstract

The annual habit in beet is due to complete or partial absence of the vernalization requirement and can cause severe problems in the beet crop. The absolute vernalization requirement in beet is controlled by a major geneB (bolting), known to be linked to the geneR (red hypocotyl color), in linkage group I. Segregation for theB andR genes was studied in several beet progenies. Penetrance of the annual habit inBb genotypes was affected by both environmental and genetic factors. The precise location in linkage group I of the major geneB was found by restriction fragment length polymorphism (RFLP) analysis in a back-cross progeny exhibiting partial penetrance of the annual habit. The linkage value betweenB andR was in good accordance with previous estimations. Use of the closest RFLP marker (pKP591: 3.8 recombination units) allowed us to estimate the penetrance of the annual habit in this back-cross as 0.62. Evidence of pseudo-compatibility was found in the wild coastal beet (Beta vulgaris sspmaritima) used as the mother plant of the back-cross: the selfing rate was estimated as 7%.

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Author notes

  1. K. Pillen

    Present address: Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853, Ithaca, New York, USA

  2. C. Jung

    Present address: Institut für Pflanzenbau und Pflanzenzüchtung, Universität Kiel, Olshausenstraße 40, D-24118, Kiel, Germany

Authors and Affiliations

  1. Laboratoire de Génétique et Evolution des Populations Végétales, URA-CNRS 1185, Bât. SN2, Université de Lille 1, 59655, Villeneuve d'Ascq Cedex, France

    P. Boudry, P. Saumitou-Laprade & H. Van Dijk

  2. Botanisches Institut der Ludwig-Maximilians-Universität München, Menzinger Straße 67, D-80638, München, Germany

    R. Wieber, K. Pillen & C. Jung

Authors
  1. P. Boudry
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  2. R. Wieber
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  3. P. Saumitou-Laprade
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  4. K. Pillen
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  5. H. Van Dijk
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  6. C. Jung
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Communicated by R. Hagemann

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Boudry, P., Wieber, R., Saumitou-Laprade, P. et al. Identification of RFLP markers closely linked to the bolting gene B and their significance for the study of the annual habit in beets (Beta vulgaris L.). Theoret. Appl. Genetics 88, 852–858 (1994). https://doi.org/10.1007/BF01253996

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  • DOI: https://doi.org/10.1007/BF01253996

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