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Genetic transformation and regeneration of transgenic plants in grapevine (Vitis rupestris S.)

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Abstract

Isolated somatic embryos from petiole-derived callus cultures ofVitis rupestris Scheele have been employed in experiments on genetic transformation. Co-cultivation of somatic embryos during embryogenesis induction withAgrobacterium tumefaciens strain LBA4404, which contains the plasmid pBI121 carrying the neomycin phosphotranspherase and theβ-glucuronidase genes, produced transformed cellular lines capable of recurrent somatic embryogenesis. Precocious selection for high levels of kanamycin (100 mgl-1) was an important part of our transformation protocol. Transformed lines still have strongβ-glucuronidase expression as well as stable insertion of the marker genes after 3 years of in-vitro culture, during which they have maintained their capacity to organize secondary embryos and to regenerate transgenic plants with an agreeable efficiency (13%).

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Authors and Affiliations

  1. Istituto Agrario, via Mach 1, 38010, San Michele all'Adige, Trento, Italy

    L. Martinelli

  2. Istituto Sperimentale per le Colture Industriali, Bologna, Italy

    G. Mandolino

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  1. L. Martinelli
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  2. G. Mandolino
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Communicated by G. Wenzel

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Martinelli, L., Mandolino, G. Genetic transformation and regeneration of transgenic plants in grapevine (Vitis rupestris S.). Theoret. Appl. Genetics 88, 621–628 (1994). https://doi.org/10.1007/BF01253963

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  • DOI: https://doi.org/10.1007/BF01253963

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