Résumé
Un Interféron partiellement purifié par précipitation avec CH3 COO2Zn inhibe la synthèse de l'ADN et la formation des virions du virus vaccinal sur cellules L. Ces deux effets sont proportionnels à la concentration d'interféron utilisée. L'utilisation de fortes multiplicités d'infection permet de dissocier l'effet inhibiteur de l'interféron sur les événements précoces du cycle de développement (synthèse de l'ADN viral) de celui sur les événements tardifs (formation des virions). 6 heures de prétraitement, dont 4 à 37°C, sont nécessaires pour observer l'effet inhibiteur maximum. Au cours de la phase de prétraitement le contact entre l'interféron et les cellules doit être au minimum de 4 h 30.
Summary
An interferon, partially purified by precipitation with. CH3COO2Zn, was found to inhibit DNA synthesis as well as viral-particle formation of vaccinia virus on L cells. This inhibition was proportional to the dose of Interferon. When high multiplicities of virus were used, it was possible to dissociate the inhibiting action of interferon on early events of the development cycle (viral DNA synthesis) from inhibition on later events (viral-particle formation). A 6-hour pretreatment, including a four-hour incubation at 37° C, was necessary to exhibit an optimal inhibiting action. During pretreatment, contact of cells with interferon had to be maintained 4.30 hours at least.
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Bibliographie
Taylor, J.: Studies on the mechanism of action of interferon. I. Interferon action on RNA synthesis in chick embryo fibroblasts infected with Semliki Forest virus. Virology25, 340–349 (1965).
Cocito, C., E. De Mayer, andP. De Somer: The action of interferon on the synthesis of RNA in fibroblasts infected with a RNA-virus. Life Science1, 753–758 (1962).
De Somer, P., A. Prinzie, P. Denys, Jr., andE. Schonne: Mechanism of action of interferon. I. Relationship with viral ribonucleic acid. Virology16, 63–70 (1962).
Grossberg, S. E., andJ. J. Holland: Interferon and viral ribonucleic acid. Effect on virus susceptible and insusceptible cells. J. Immunol.88, 708–714 (1962).
Lockart, R. Z., Jr., T. Sreevalsan, andB. Hozn: Inhibition of viral RNA synthesis by interferon. Virology18, 493 (1962).
Levy, H. B., Leroy F. Snellbaker, andS. Baron: Studies on the mechanism of action of interferon. Virology21, 48–55 (1963).
Ghosh, S. N., andG. E. Gifford: Effect of interferon on the dynamics of H3 thymidine incorporation and thymidine kinase induction in chick fibroblast cultures infected with vaccinia virus. Virology27, 186–192 (1965).
Ohno, S., andT. Nozima: Mode of action of interferon on the early stage of vaccinia virus multiplication in chick embryo fibroblasts. Acta virol.10, 310–314 (1966).
Levine, S., W. E. Magee, R. D. Hamilton, andO. V. Miller: Effect of Interferon on early enzyme and viral DNA synthesis in vaccinia virus infection. Virology32, 33–40 (1967).
Joklik, W. K.: Mechanism of action of interferon, p. 110–127. In:G. E. W. Wolstenholme etMaeve O'Connor (ed.). Interferon. Ciba Foundation Symposium. London: 1968. J. & A. Churchill Ltd.
Marcus, P. I., andJ. M. Salb: Molecular basis of interferon action: inhibition of viral RNA translation. Virology30, 502–516 (1966).
Levine, S.: Effect of actinomycin D and puromycin dihydrochloride on action of interferon. Virology24, 586–588 (1964).
Friedman, R. M., andJ. A. Sonnabend: Inhibition of interferon action by puromycin. J. Immunol.95, 696–703 (1965).
Taylor, J.: Inhibition of interferon action by actinomycin. B.B.R.C.14, 447–451 (1964).
Levine, S.: Persistence of active interferon in cells washed after treatment with interferon. Proc. Soc. exp. Biol. (N.Y.)121, 1041–1045 (1966).
Friedman, R. M.: Interferon Binding: The first step in establishment of antiviral activity. Science156, 1760–1761 (1967).
Vilček, J., andD. E. Lowy: Interaction of interferon with chick embryo cells. Arch. ges. Virusforsch.21, 253–264 (1967).
Pokidova, N. V., N. M. Furer, G. A. Sapozhnikova, andZ. V. Ermol'eva: Purification of interferon by chromatography on Sephadex CM. Antibiotiki10, 713 (1965).
Wagner, R. R.: Biological studies of interferon. I. Suppression of cellular infection with eastern equine encephalomyelitis virus. Virology13, 323–337 (1961).
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Ce travail a bénéficié d'une aide de la Direction des Recherches et des Moyens d'Essais — Contrat D.R.M.E. no 67-34-142-00-480-75-01.
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Keith, G., Peter, R., Guir, J. et al. Conditions expérimentales de l'inhibition de la synthese de l'ADN et de la formation des virions du virus vaccinal par un interféron partiellement purifié. Archiv f Virusforschung 27, 32–43 (1969). https://doi.org/10.1007/BF01250312
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DOI: https://doi.org/10.1007/BF01250312