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Characterization and distribution of two new foamy viruses isolated from chimpanzees

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Summary

Pan 1 and Pan 2 viruses are persistent viruses recoverable from many organs, including brain, of most chimpanzees; both may be present in the same organ of a given animal. These viruses induce a vacuolated, foamy syncytia without inclusion bodies both in chimpanzee explant cultures and in primary human embryo kidney (HEK) cells. Both viruses are not inhibited by IUDR. are chloroform and pH 3.0 sensitive and are inactivated following exposure to 45° C for 30 minutes. Pan 2 virus, the only one tested, is sensitive to visible light. Neither virus has yielded a detectable hemagglutinin, nor has hemadsorption of guinea pig RBC been observed. Direct and blind passage inoculations have not induced infection or evidence of viral replication in small laboratory animals. By electron microscopy they are indistinguishable from each other and are approximately 125 nm in diamter. Viral antigens are demonstrable inin vitro cultures of chimpanzee tissues as well as infected HEK cells by direct fluorescent antibody staining.

Pan 1 and Pan 2 viruses do not share common antigens detectable by neutralization or fluorescent antibody staining. The viruses are not neutralized by reference reagent antisera specific for simian foamy virus types 1, 3, 4, 5, measles, canine distemper or other myxo- and pseudomyxoviruses tested. Antiserum prepared against foamy virus type 2, at a dilution of 1∶10, neutralizes 50 TCID50 of Pan 2 virus, whereas antiserum prepared against Pan 2 virus does not neutralize foamy virus, type 2. Homologous antibody is present in the serum of each chimpanzee from whose tissue the virus has been recovered. Antibodies to the Pan foamy viruses have been detected in uninoculated control chimpanzees and in those bled in the African bush.

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References

  1. Basnight, M., Jr., N. G. Rogers, C. J. Gibbs, Jr., andD. C. Gajdusek: Characterization of four new adenovirus serotypes isolated from chimpanzee tissue explants. Amer. J. Epidem.94, 166–171 (1971).

    Google Scholar 

  2. Carski, T.: A fluorescent antibody study of the simian foamy agent. J. Immunol.84, 426–433 (1960).

    Google Scholar 

  3. Cherry, W. B., M.Goldman, and T. R.Carski: Fluorescent antibody techniques in the diagnosis of communicable diseases. U.S. publ. Hlth Serv. Publ. 729 (1961).

  4. Clarke, J. K., andJ. T. Attridge: The morphology of simian foamy agents. J. gen. Virol.3, 185–190 (1968).

    Google Scholar 

  5. Clarke, J. K., J. T. Attridge, andF. W. Gay: The morphogenesis of simian foamy agents. J. gen. Virol.4, 183–188 (1969).

    Google Scholar 

  6. Clarke, J. K., F. W. Gay, andJ. T. Attridge: Replication of simian foamy virus in monkey kidney cells. J. Virol.3, 358–362 (1969).

    Google Scholar 

  7. Coons, A. H., andM. H. Kaplan: Localization of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody. J. exp. Med.91, 1–13 (1950).

    Google Scholar 

  8. Cutchins, E. C., andT. R. Dayhoff: Photoinactivation of measles virus. Virology17, 420–425 (1962).

    Google Scholar 

  9. Eagle, H.: Nutritional needs of mammalian cells in tissue culture. Science122, 501–504 (1955).

    Google Scholar 

  10. Feldman, H., andS. Wang: Sensitivity of various viruses to chloroform. Proc. Soc. exp. Biol. (N.Y.)106, 736–740 (1961).

    Google Scholar 

  11. Fleming, W. A., andJ. K. Clarke: Fluorescence assay of foamy viruses. J. gen. Virol.6, 277–284 (1970).

    Google Scholar 

  12. Gajdusek, D. C., C. J. Gibbs, Jr., andM. Alpers: Experimental transmission of a kuru-like syndrome to chimpanzees. Nature (Lond.)209, 794–796 (1966).

    Google Scholar 

  13. Gajdusek, D. C., N. G. Rogers, M. Basnight, C. J. Gibbs, Jr., andM. Alpers: Transmission experiments with kuru in chimpanzees and the isolation of latent viruses from the explanted tissues of affected animals. Ann. N.Y. Acad. Sci.162, 529–550 (1969).

    Google Scholar 

  14. Gibbs, C. J., Jr., D. C. Gajdusek, D. M. Asher, M. P. Alpers, E. Beck, P. M. Daniel, andW. B. Matthews: Creutzfeldt-Jakob disease (spongiform encephalopathy): transmission to the chimpanzee. Science161, 388–389 (1968).

    Google Scholar 

  15. Hamparian, V. V., M. R. Hilleman, andA. Ketler: Contributions to characterization and classification of animal viruses. Proc. Soc. exp. Biol. (N.Y.)112, 1040 to 1050 (1963).

    Google Scholar 

  16. Hull, R.: The Simian Viruses. Virology Monograph, No. 2. pp. 1–142, Wien-New York: Springer-Verlag, 1968.

    Google Scholar 

  17. Johnston, P.: A second immunologic type of simian foamy virus: monkey throat infections and unmasking by both types. J. infect. Dis.109, 1–9 (1961).

    Google Scholar 

  18. Johnston, P.: Taxonomic features of seven serotypes of simian and ape foamy viruses. Infection & Immunity3, 793–799 (1971).

    Google Scholar 

  19. Parks, W., G. Todaro, E. Scolnick, andS. Aaronson: RNA dependent DNA polymerase in primate syncytium-forming (foamy) viruses. Nature (Lond.)229, 258–260 (1971).

    Google Scholar 

  20. Plummer, G.: Foamy virus of monkeys. J. gen. Microbiol.29, 703–709 (1962).

    Google Scholar 

  21. Riggs, J. L., R. J. Seiwald, J. Burckhalter, C. M. Downs, andT. F. Metcalf: Isothiocyanate compounds as fluorescent labeling agents for immune serum. Amer. J. Path.34, 1081–1097 (1958).

    Google Scholar 

  22. Rogers, N. G., M. Basnight, C. J. Gibbs, Jr., andD. C. Gajdusek: Latent viruses in chimpanzees with experimental kuru. Nature (Lond.)216, 446–449 (1967).

    Google Scholar 

  23. Ruckle, G.: Studies with the monkey-intra-nuclear-agent (MINIA) and foamyagent derived from spontaneously degenerating monkey kidney cultures. I. Isolation and tisuse culture behavior of the agents and identification of MINIA as closely related to measles virus. II. Immunologic and epidemiologic observations in monkeys in a laboratory colony. Arch. ges. Virusforsch.8, 139–166, 167–182 (1958).

    Google Scholar 

  24. Stiles, G. E., J. L. Bittle, andU. J. Cabasso: Comparison of simian foamy virus strains including a new serological type. Nature (Lond.)201, 1350 (1964).

    Google Scholar 

  25. Thormar, H.: Effect of 5-bromodeoxyuridine and actinomycin D on the growth of visna virus cell cultures. Virology26, 36–43 (1965).

    Google Scholar 

  26. Tyrrell, D. A. J., andR. M. Chanock: Rhinoviruses: a description. Science141, 152–153 (1963).

    Google Scholar 

  27. Vogel, J., andA. Shelokov: Adsorption-hemagglutination test for influenza virus in monkey kidney tissue culture. Science126, 358–359 (1957).

    Google Scholar 

  28. Weller, T. H., andA. H. Coons: Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro. Proc. Soc. exp. Biol. (N.Y.)86, 789–794 (1954).

    Google Scholar 

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This work was submitted as partial fulfillment for the Ph.D. degree at The Catholic University of America, Washington, D.C.

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Hooks, J.J., Gibbs, C.J., Cutchins, E.C. et al. Characterization and distribution of two new foamy viruses isolated from chimpanzees. Archiv f Virusforschung 38, 38–55 (1972). https://doi.org/10.1007/BF01241354

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