Abstract
We examined the effect of acetylsalicylic acid (ASA) onn-butyl-(4-hydroxybutyl)nitrosamine (BHBN)-induced bladder carcinogenesis in male Wistar rats. Of 29 rats that received 0.05% BHBN in their drinking water for 9 weeks, 8 developed bladder cancer. Only 1 out of 29 rats that received 0.1% ASA in their diet for 20 weeks, including the period of BHBN consumption, developed a tumor. That difference is statistically significant. Bladder weight was significantly higher in rats given BHBN than in controls and in rats given both BHBN and ASA. We conclude that ASA inhibits BHBN-induced bladder carcinogenesis.
Similar content being viewed by others
Abbreviations
- ASA:
-
acetylsalicylic acid
- BHBN:
-
n-butyl-(4-hydroxybutyl)nitrosamine
- FANFT:
-
n-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide
- NSAID:
-
nonsteroidal anti-inflammatory drug
References
Danon A, Zenser TV, Thomasson DL, Davis BB (1986) Eicosanoid synthesis by cultured human urothelial cells: potential role in bladder cancer. Cancer Res 46:5676–5681
Droller MJ, Gomolka D (1984) Indornethacin and poly I:C in the inhibition of careinogen-induced bladder cancer in an experimental animal model. J Urol 131:1212–1217
Fujita J, Ohuchi N, Ito N, Reynolds SH, Yoshida O, Nakayama H, Kitamura Y (1988) Activation of H-ras-oncogene in rat bladder tumors induced byn-butyl-n-(4-hydroxybutal)nitrosamine. JNCI 80:37–43
Greenwald P, Malone WF, Stern HR (1990) Concepts in cancer chemoprevention research. Cancer 65:1483–1490
Ito N, Hiasa Y, Tamai A, Okajima E, Kitamura H (1969) Histogenesis of urinary bladder tumors induced byn-butyl-n-(4-hydroxybutyl)nitrosamine in rats. Gann 60:401–410
Jeremy JY, Mikhailidis DP, Dandona P (1985) Cigarette smoke extracts inhibit prostacyclin synthesis by the rat urinary bladder. Br J Cancer 51:837–842
Lockwood HA, Murphy SK, Borislow S, Lazarus A, Pendergast M (1987) Cellular signal transduction and the reversal of malignancy. J Cell Biochem 33:237–255
Malone WF, Kellhof GJ, Pierson H, Greenwald P (1987) Chemoprevention of bladder cancer. Cancer 60:650–657
Matanoski GM, Elliott EA (1981) Bladder cancer epidemiology. Epidemiol Rev 3:203–229
Mattammal MB, Zenser TV (1981) Prostaglandin hydroperoxidasemediated 2-amino-4-(5-nitro-2-furyl)-14C-thiazole metabolism and nucleic acid binding. Cancer Res 41:4961–4966
McCormick DL, Wilson AM (1986) Combination chemoprevention of rat mammary carcinogenesis by indimethacin and butylated hydroxytoluene. Cancer Res 46:3907–3911
Murasaki G, Zenser TV, Davis BB, Cohen SM (1984) Inhibition by aspirin ofn-(4-(5-nitro-2-furyl)-thiazolyl)formamide-induced bladder carcinogenesis and enhancement of forestomach carcinogenesis. Carcinogeneseis 5:53–55
Narisawa T, Sato M, Tani M, Kudo T, Takahashi T, Goto A (1981) Inhibition of development of methylnitrosourea-induced rat colon tumors by indomethacin treatment. Cancer Res 41:1954–1957
Reddy BS, Maruyama H, Kelloff G (1987) Dose-related inhibition of colon carcinogenesis by dietary piroxicam, a nonsteroidal antiinflammatory drug, during different stages of rat colon tumor development. Cancer Res 47:5340–5346
Rehn L (1895) Blasengeschwülste bei Fuchsinarbeitern. Arch Clin Chir 50:588–600
Rosenberg L, Palmer JR, Zauber AG, Warshauer ML, Stolley PD, Shapiro S (1991) A hypothesis: nonsteroidal antiinflammatory drugs reduce the incidence of large-bowel cancer. JNCI 83:355–358
Sakata T, Hasegawa R, Johannson SL, Zenser TV, Cohen SM (1986) Inhibition by aspirin ofn-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide initiation and sodium saccharin promotion of urinary bladder carcinogenesis in male F344 rats. Cancer Res 46:3906–3909
Sawczuk IS, Walsh W, King W, Olsson CA, Nguyen-Huu C (1987) Enhanced expression of harvey-Ras oncogene in FANFT-induced transitional cell carcinoma. Urol Int 42:321–325
Studer UE, Biedermann C, Chollet D, Karrer P, Kraft R, Toggenburg H, Vonbank F (1984) Prevention of recurrent superficial bladder tumors by oral etretinate: preliminary results of a randomized, double blind multicenter trial in Switzerland. J Urol 131:47–50
Thun MJ, Namboodiri MM, Heath CW Jr (1991) Aspirin use and reduced risk of fatal colon cancer. N Engl J Med 325:1593–1596
Viola MV, Fromowitz F, Rravez S, Deb S, Schlom J (1985)ras oncogene p21 expression is increased in premalignant lesions and high grade bladder carcinoma. J Exp Med 161:1213–1218
Wallace DMA (1988) Occupational urothelial cancer. Br J Urol 61:175–182
Weinstein IB (1988) The origins of human cancer: molecular mechanisms of carcinogenesis and their implications for cancer prevention and treatment. Twenty-seventh G.H.A. Clowes Memorial Award Lecture. Cancer Res 48:4135–4143
Zenser TV, Mattammal MB, Wise RW, Rice JR, Davis BB (1983a) Prostaglandin H synthase-catalyzed activation of benzidine: a model to assess pharmacologic intervention in the initiation of chemical carcinogenesis. J Pharmacol Exp Ther 227:545–550
Zenser TV, Palmier MO, Mattammal B, Bolla RI, Davis BB (1983b) Comparative effect of prostaglandin H synthase-catalyzed binding of two 5 nitrofuran urinary bladder carcinogens. J Pharmacol Exp Ther 227:139–143
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Klän, R., Knispel, H.H. & Meier, T. Acetylsalicylic acid inhibition of n-butyl-(4-hydroxybutyl) nitrosamine-induced bladder carcinogenesis in rats. J Cancer Res Clin Oncol 119, 482–485 (1993). https://doi.org/10.1007/BF01215929
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01215929