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Elevation of ratio of urinary N-acetylneuraminlactose to free sialic acid in some advanced cancer patients

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Abstract

We estimated the levels of free sialic acid and sialylated oligosaccharides excreted in the urine of normal donors (n=10) and patients with gastric cancer (n=6) and colorectal cancer (n=4). The total sialic acid level in cancer patients was similar to that in normal donors. However, the ratios of glycosidically bound sialic acids to free sialic acid were higher in some advanced cancer patients than in the normal donors. A major component of sialylated oligosaccharides was N-acetylneuraminyl α (2 → 3) lactose. The elevation of the urinary ratio of this sialylated oligosaccharide to free sialic acid observed in some advanced cancer patients in this study may reflect the elevation of sialyltransferase activity in tumor tissues.

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References

  1. Erbil KM, Jones JD, Klee GG. Use and limitations of serum total and lipid-bound sialic acid concentrations as markers for colorectal cancer. Cancer 1985;55:404–409.

    Google Scholar 

  2. Silver HKB, Rangel DM, Morton DL, Serum sialic acid elevations in malignant melanoma patients. Cancer 1978;41:1497–1499.

    Google Scholar 

  3. Magnani JL, Nilsson B, Brockhaus M, et al. A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II. j Biol Chem 1982;257:14365–14369.

    Google Scholar 

  4. Atkinson BF, Ernst CS, Herlyn M, et al. Gastrointestinal cancer-associated antigen in immunoperoxidase assay. Cancer Res 1982;42:4820–4823.

    Google Scholar 

  5. Fukushima K, Hirota M, Terasaki PI, et al. Characterization of sialosylated Lewisx as a new tumor-associated antigen. Cancer Res 1984;44:5279–5285.

    Google Scholar 

  6. Kessel D, Allen J. Elevated plasma sialyltransferase in the cancer patient. Cancer Res 1975;35:670–672.

    Google Scholar 

  7. Berg EL, Robinson MK, Mansson O, et al. A carbohydrate domain common to both sialyl Lea and sialyl Lex is recognized by the endothelial cell leukocyte adhesion molecule ELAM-1. J Biol Chem 1991;266:14869–14872.

    Google Scholar 

  8. Lundblad A, Hallgren P, Rudmark A, et al. Structures and serological activities of three oligosaccharides isolated from urines of nonstarved secretors and from secretors on lactose diet. Biochemistry 1973;12:3341–3345.

    Google Scholar 

  9. Lundblad A. Urinary amino acid glycosides and oligosaccharides in health and disease. Scand J Clin Lab Invest 1980;40[Suppl 154]:3–11.

    Google Scholar 

  10. Araki H, Yamada M. Sialic acid. In: Bergmeyer HU (ed) Methods of enzymatic analysis. 3rd ed., vol. VI, Metabolites 1: Carbohydrates. Weinheim: Verlag Chemie, 1984;80–90.

    Google Scholar 

  11. Parkkinen J, Finne J. Isolation of sialyl oligosaccharides and sialyl oligosaccharide phosphates from bovine colostrum and human urine. Methods Enzymol 1987;138:289–300.

    Google Scholar 

  12. Huttunen JK. Neuraminic acid-containing oligosaccharides of human urine. Ann Med Exp Biol Fenn 1966;44[Suppl 12]:1–60.

    Google Scholar 

  13. Shukla AK, Schauer R. Analysis of N,O-acylated neuraminic acids by high-performance liquid anion-exchange chromatography. J Chromatogr 1982;244:81–89.

    Google Scholar 

  14. Veh RW, Michalski JC, Corfield AP, et al. New Chromatographic system for the rapid analysis and preparation of colostrum sialyloligosaccharides. J Chromatogr 1981;212:313–322.

    Google Scholar 

  15. Jourdian GW, Dean L, Roseman S. The sialic acids. J Biol Chem 1971;246:430–435.

    Google Scholar 

  16. Hakomori S. A rapid permethylation of glycolipid, and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide. J Biochem 1964;55:205–208.

    Google Scholar 

  17. Hoff SD, Irimura T, Matsushita Y, et al. Metastatic potential of colon carcinoma. Arch Surg 1990;125:206–209.

    Google Scholar 

  18. Itai S, Arii S, Tobe R, et al. Significance of 2–3 and 2–6 sialylation of Lewis A antigen in pancreas cancer. Cancer 1988;61:775–787.

    Google Scholar 

  19. Dube VE. The structural relationship of blood group-related oligosaccharides in human carcinoma to biological function: A perspective. Cancer Metastasis Rev 1987;6:541–557.

    Google Scholar 

  20. Gold P, Freedman SO. Demonstration of tumor-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques. J Exp Med 1964;121:439–462.

    Google Scholar 

  21. Hallgren P, Lundblad A. Structural analysis of nine oligosaccharides isolated from the urine of a blood group 0, nonsecretor, woman during pregnancy and lactation. J Biol Chem 1977;252:1014–1022.

    Google Scholar 

  22. Harvey BE, Toth CA, Wagner HE, et al. Sialyltransferase activity and hepatic tumor growth in a nude mouse model of colorectal cancer metastases. Cancer Res 1992;52:1775–1779.

    Google Scholar 

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Authors and Affiliations

  1. Second Department of Surgery, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-01, Toyama, Japan

    Ichiroh Shimada & Takashi Sakamoto

  2. Department of Radiological Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-01, Toyama, Japan

    Miki Shoji & Ryusuke Futatsuya

  3. Department of Public Health, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-01, Toyama, Japan

    Terutaka Katoh

  4. Department of Legal Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-01, Toyama, Japan

    Yoshihiko Kominato

  5. Department of Legal Medicine, Faculty of Medicine, Fujita Health University School of Medicine, 1–98 Dengakugakubo Kutsukake-cho, 470-11, Toyoake, Japan

    Takashi Fujikura

Authors
  1. Ichiroh Shimada
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  2. Miki Shoji
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  3. Ryusuke Futatsuya
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  4. Terutaka Katoh
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  5. Yoshihiko Kominato
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  6. Takashi Sakamoto
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  7. Takashi Fujikura
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Shimada, I., Shoji, M., Futatsuya, R. et al. Elevation of ratio of urinary N-acetylneuraminlactose to free sialic acid in some advanced cancer patients. J Gastroenterol 30, 21–27 (1995). https://doi.org/10.1007/BF01211370

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  • DOI: https://doi.org/10.1007/BF01211370

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