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Protoplasts from the cyanobacterium,Spirulina platensis

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Abstract

Protoplasts were obtained from the filamentous blue-green algaSpirulina platensis by treating the filaments with 0.05% (w/v) lysozyme in 0.03m phosphate buffer. The protoplasts regenerated cell walls and formed colonies when plated on a regeneration medium. The highest percentage of regeneration, 40% was obtained after 21 days.

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Authors and Affiliations

  1. Botany Department, Faculty of Science, Tanta University, Tanta, Egypt

    A. M. Abo-Shady, S. M. Abou-El-Souod, Abd El-Raheem, R. El-Shanshoury & Y. A. G. Mahmoud

Authors
  1. A. M. Abo-Shady
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  2. S. M. Abou-El-Souod
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  3. Abd El-Raheem
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  4. R. El-Shanshoury
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  5. Y. A. G. Mahmoud
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Abo-Shady, A.M., Abou-El-Souod, S.M., El-Raheem, A. et al. Protoplasts from the cyanobacterium,Spirulina platensis . World J Microbiol Biotechnol 8, 385–386 (1992). https://doi.org/10.1007/BF01198750

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  • DOI: https://doi.org/10.1007/BF01198750

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