Summary
The β-galactosidase gene ofStreptococcus thermophilus was cloned into plasmid vector, pVT100-U, and used to transform a strain ofEscherichia coli andSaccharomyces cerevisiae. Transformants which expressed β-galactosidase activity were obtained in bothE. coli andSaccharomyces cerevisiae, the highest activity found in a yeast recombinant. The expression and thermostability of the cloned β-galactosidase genes from different plasmid constructions were compared with the streptococcal β-galactosidase. The recombinant protein was equivalent to the specific activity and thermostability ofS. thermophilus.
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Lee, B.H., Robert, N., Jacques, C. et al. Cloning and expression of β-galactosidase gene from Streptococcus thermophilus in Saccharomyces cerevisiae. Biotechnol Lett 12, 499–504 (1990). https://doi.org/10.1007/BF01086342
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DOI: https://doi.org/10.1007/BF01086342