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Diurnal rhythmicity of absorption of a lipid compound (vitamin K-1)in vivo in the rat

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Abstract

The possibility of rhythmicity in the intestinal absorption of lipids was explored by assessing the absorption of vitamin K-1 by the unanesthetized rat at 6pm, 12 and 6am, and 12pm. A marked variability in the absorption rate of vitamin K-1 was found throughout the 18-hr period. The highest rates of absorption occurred at midnight (139.8±.22 and 134.4±9.1 pmol/min/10 cm of jejunum and ileum, respectively). The lowest rates of absorption occurred at 6am (54.5±1 and 81.4±7.4 pmol/min/10 cm of jejunum and ilenum, respectively). Absorption rates at noon were not different from absorption at 6am but an initial increase in absorption was noted at 6pm. Synchronization of the absorptive rate with time is most likely related to the time of feeding and not to changes in the pattern of illumination. The possibility of marked diurnal variability in the absorption rate should be considered in the design and execution of intestinal absorption experiments.

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References

  1. Feigin RD, Dangerfield HG, Beisel WR: Circadian periodicity of blood amino acids in normal and adrenalectomized mice. Nature (London) 221:94–95, 1969

    Google Scholar 

  2. Baril EF, Potter VR: Systemic oscillations of amino acid transport in liver from rats adapted to controlled feeding. J Nutr 95:228–237, 1968

    Google Scholar 

  3. Wurtman RJ, Shoemaker WJ, Larin F: Mechanism of the daily rhythm in hepatic tyrosine transaminase activity: Role of dietary tryptophan. Proc Natl Acad Sci USA 800–807, 1968

  4. Cohn C, Webb L, Joseph D: Diurnal rhythms in urinary electrolyte excretions by the rat. Influence of feeding habits. Life Sci 9:803–809, 1971

    Google Scholar 

  5. Furuya S, Yugari Y: Daily rhythmic chance in the transport of histidine by everted sacs of rat small intestines. Biochim Biophys Acta 241:245–248, 1971

    PubMed  Google Scholar 

  6. Furuya S, Yugari Y: Daily rhythmic change of L-histidine and glucose absorptions in rat small intestinein vivo. Biochim Biophys Acta 343:558–564, 1974

    PubMed  Google Scholar 

  7. Stevenson NR, Fierstein JS: Circadian rhythms of intestinal sucrase and glucose transport: Cued by time of feeding. Am J Physiol 230(3):731–735, 1976

    PubMed  Google Scholar 

  8. Stevenson NR, Ferrigni F, Parnicky K, Day S, Fierstein JS: Effect of changes in feeding schedule on diurnal rhythms and daily activity levels of intestinal brush border enzymes and transport systems. Biochim Biophys Acta 406:131–145, 1975

    PubMed  Google Scholar 

  9. Hollander D: Vitamin K-1 absorption by everted intestinal sacs of the rats. Am J Physiol 225:360–364, 1973

    PubMed  Google Scholar 

  10. Hollander D, Rim E, Muralidhara KS: Vitamin K-1 intestinal absorption in vivo: Influence of luminal contents on transport. Am J Physiol 232(1):E69-E74, 1977

    PubMed  Google Scholar 

  11. Hollander D, Rim E: Effect of luminal constituents on vitamin K-1 absorption into thoracic duct lymph. Am J Physiol 234(1):E54-E59, 1978

    PubMed  Google Scholar 

  12. Matschiner JR, Taggart WV, Amelotti JM: The vitamin K content of beef liver. Detection of a new form of vitamin K. Biochemistry 6:1243–1248, 1967

    PubMed  Google Scholar 

  13. Dunphy PJ, Brodie AF: The structure and function of quinones in respiratory metabolism. Methods in Enzymology. DB McCormick, LD Wright (eds.). New York, Academic Press, 1971, p 422

    Google Scholar 

  14. Gregg JS: New solvent systems for thin-layer chromatography of bile acids. J Lipid Res 7:579–581, 1966

    PubMed  Google Scholar 

  15. Umbreit WW, Burris RH, Stauffer JF: Manometric and Biochemical Techniques (5th ed). Minneapolis, Burgess, 1972, p 146

    Google Scholar 

  16. Hendry EB: Osmolarity of human serum and of chemical solutions of biologic importance. Clin Chem 7:156–164, 1961

    Google Scholar 

  17. Miller DL, Schedl HP: Total recovery studies of non-absorbable indicators in the rat small intestine. Gastroenterology 58:40–46, 1967

    Google Scholar 

  18. Sokal RA, Rohlf RJ: Biometry. San Francisco, WH Freeman, 1969, pp 448–468

    Google Scholar 

  19. Al-Dewachi HS, Wright NA, Appleton DR, Watson AJ: Studies on the mechanism of diurnal variation of proliferative indices in the small bowel mucosa of the rat. Cell Tissue Kinet 9:459–467, 1976

    PubMed  Google Scholar 

  20. Saito M, Murakami E, Suda M: Circadian rhythms in disaccharidases of rat small intestine and its relation to food intake. Biochim Biophys Acta 421:177–179, 1976

    PubMed  Google Scholar 

  21. Sitren H, Stevenson NR: Rhythmic fluctuations in small intestinal functions, insulin, and gastrin in orally and intravenously fed rats. Gastroenterology 72:1133, 1977

    Google Scholar 

  22. Nishida T, Saito M, Suda M: Parallel between circadian rhythms of intestinal disaccharidases and food intake of rats under constant lighting conditions. Gastroenterology 74:224–227, 1978

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Gastroenterology, Wayne State University, 48201, Detroit, Michigan

    Daniel Hollander MD, Michael Kielb MS & Elena Rim MS

  2. Harper Hospital, 48201, Detroit, Michigan

    Daniel Hollander MD, Michael Kielb MS & Elena Rim MS

Authors
  1. Daniel Hollander MD
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  2. Michael Kielb MS
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  3. Elena Rim MS
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Hollander, D., Kielb, M. & Rim, E. Diurnal rhythmicity of absorption of a lipid compound (vitamin K-1)in vivo in the rat. Digest Dis Sci 23, 1125–1128 (1978). https://doi.org/10.1007/BF01072889

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