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Comparison of enzyme-linked immunosorbent assay and high-performance liquid chromatography for the analysis of atrazine in water from Czechoslovakia

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Abstract

Twenty-two water samples from Bohemian Paradise in Czechoslovakia were analyzed for atrazine (2-chloro-4-ethylamino-6-isopropylamino-l,3,5-triazine) by enzyme immunoassay (EIA) and high-performance liquid chromatography (HPLC). Three samples were drinking water, the others were creek water. The amount of atrazine found by EIA ranged from 0.2 to 12,700 ng/ml and 0.10 to 10,300 ng/ml by HPLC. Agreement between the methods was good. Nine samples containing the highest concentrations of atrazine were used to check the day-to-day reproducibility of both methods at high atrazine levels. The percent coefficients of variation (% CVs) ranged from 5.9 to 26.2 for the EIA and 1.5 to 33.2 for the HPLC. Day to day% CV values for each calibrator concentration ranged from 5.7 to 10.9 for EIA and 9.9 to 13.9 for HPLC. Sensitivity of the EIA was 0.2 ng/ml while it was 0.1 ng/ml for solid phase extraction HPLC and slightly less than 60 ng/ml for direct injection HPLC.

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References

  • Bardalaye PC, Wheeler WB (1986) Solid-phase extraction and capillary gas Chromatographic determination of triazines herbicides in water. J Environ Anal Chem 25:105–113

    Google Scholar 

  • Battista M, DiCorcia A, Marchetti M (1989) Extraction and isolation of triazine herbicides from water and vegetables by a double trap tandem system. Anal Chem 61:935–939

    PubMed  Google Scholar 

  • Brooks MW, Jenkins J, Jimenez M, Quinn T, Clark JM (1989) Rapid method for the determination of alachor, atrazine and metolachlor in ground water by solid-phase extraction. Analyst 144:405–406

    Google Scholar 

  • Brouwer ER, Lingeman H, Th. Brinkman UA (1990) Use of membrane extraction disks for on-line trace enrichment of organic compounds from aqueous samples. Chromatographia 29:415–418

    Google Scholar 

  • Bushway RJ, Perkins B, Savage SA, Lekousi SJ, Ferguson BS (1988) Determination of atrazine residues in water and soil by enzyme immunoassay. Bull Environ Contam Toxicol 40:647–654

    PubMed  Google Scholar 

  • Coppi S, Betti A (1989) Liquid Chromatographic separation and behavior of some triazines on styrene-divinylbenzene columns. J Chromatogr 472:406–410

    Google Scholar 

  • Davoli E, Benfenate E, Bagnati R, Fanelli R (1987) Analysis of atrazine in underground waters as part per trillion levels as an early warning method for contamination and for soil distribution studies. Chemosphere 16:1425–1430

    Google Scholar 

  • DiCorcia A, Marchetti M, Samperi R (1987) Rapid determination of simazine and atrazine in water at the parts per trillion level. J Chromatogr 405:357–367

    PubMed  Google Scholar 

  • Ferris IG, Haigh BM (1987) A rapid and sensitive HPLC procedure for the determination of atrazine residues in soil water extracts. J Chromatogr Sci 25:170–173

    PubMed  Google Scholar 

  • Frank R, Clegg BS, Sherman C, Chapman ND (1990) Triazine and chloroacetamide herbicides in Sydenham river water and municipal drinking water, Dresden, Ontario, Canada, 1981–1987. Arch Environ Contam Toxicol 19:319–324

    PubMed  Google Scholar 

  • Froehlich D, Meier W (1989) HPLC determination of triazines in water samples in the ppt-range by on-column trace enrichment. J High Resolut Chromatogr 12:340–342

    Google Scholar 

  • Goodrow MH, Harrison RO, Hammock BD (1990) Hapten synthesis antibody development and competitive inhibition enzyme immunoassay fors-triazine herbicides. J Agric Food Chem 38:990–996

    Google Scholar 

  • Grob K Jr, Li Z (1989) Coupled reversed-phase liquid chromatography-capillary gas chromatography for the determination of atrazine in water. J Chromatogr 473:423–430

    Google Scholar 

  • Hallberg GR (1989) Pesticide pollution of groundwater in the humid United States. Agric Ecosys Environ 26:299–367

    Google Scholar 

  • Harrison RO, Goodrow MH, Hammock BD (1991) Competitive inhibition ELIS A for thes-triazine herbicides:assay optimization and antibody characterization. J Agric Food Chem 39:122–128

    Google Scholar 

  • Huang, LQ (1989) Simultaneous determination of alachlor, metolachlor, atrazine, and simazine in water and soil by isotope dilution gas chromatography/mass spectrometry. J Assoc Offic Anal Chem 72:349–354

    Google Scholar 

  • Huber SJ (1985) Improved solid-phase enzyme immunoassay systems in the ppt range for atrazine in fresh water. Chemosphere 14:1795–1803

    Google Scholar 

  • Karlhuber BA, Hormann WD, Ramsteiner KA (1975) Pesticide residue analysis by mass fragmentography. Anal Chem 47:2450–2452

    PubMed  Google Scholar 

  • Kreindl TG, Malissa H, Winsauer K (1986) Method for the simultaneous determination of traces of atrazine, pyrazon and lindane for monitoring water supplies. Mikrochim Acta 1:1–13

    Google Scholar 

  • Lee H, Chau ASY (1983) Determination of trifluralin, diallate, atrazine, barban, diclofop-methyl and benzolpropethyl in natural waters at parts per trillion levels. J Assoc Offic Anal Chem 66:651–658

    Google Scholar 

  • Leistra M, Boesten, JJTI (1989) Pesticide contamination of groundwater in Western Europe. Agric Ecosys Environ 26:369–389

    Google Scholar 

  • Lopez-Avila V, Hirata P, Kraska S, Flanagan M, Taylor JH Jr (1985) Determination of atrazine, lindane, pentachlorophenol, and diazinon in water and soil by isotope dilution gas chromatography/mass spectrometry. Anal Chem 57:2797–2801

    Google Scholar 

  • Mangani F, Burner F (1983) Sample enrichment and GC-MS determination of herbicides in water. Chromatographia 17:377–380

    Google Scholar 

  • Mangani F, Crescentini G, Palma P, Bruner F (1988) Performance of graphitized carbon black cartridges in the extraction of some organic piority pollutants from water. J Chromatogr 452:527–534

    Google Scholar 

  • Pereira WE, Rostad CE, Leiker TJ (1990) Determination of trace levels of herbicides and their degradation products in surface and ground waters by gas chromatography/ion-trap mass spectrometry. Anal Chim Acta 228:69–75

    Google Scholar 

  • Popl M, Voznakova Z, Tatar V (1983) Determination of triazines in water by GC and LC. J Chromatogr Sci 21:39–42

    Google Scholar 

  • Ramsteiner KA (1989) Automated on-line trace enrichment and determination of organic compounds in ground and tap water by HPLC. J Chromatogr 465:410–416

    Google Scholar 

  • Rivera J, Caixach J, deTorres M, Ventura F (1986) Fate of atrazine and trifluralin from an industrial waste dumping at the Llobregat River. Int J Environ Anal Chem 24:183–191

    PubMed  Google Scholar 

  • Schlaeppi JM, Fory W, Ramsteiner K (1989) Hydroxyatrazine and atrazine determination in soil and water by enzyme-linked immunosorbent assay using specific monoclonal antibodies. J Agric Food Chem 37:1532–1538

    Google Scholar 

  • Sherma J (1986) Determination of triazine and chlorphenoxy acid in natural water samples by solid phase extraction and quantitative thin layer chromatography. J Liq Chromatogr 9:3433–3438

    Google Scholar 

  • Smolkova E, Pacakova V (1978) Liquid Chromatographie separation and behavior of some substituted s-triazines in a CN bonded stationary phase. Chromatographia 11:698–701

    Google Scholar 

  • Steinheimer TR, Brooks MG (1984) Development and evaluation of a gas Chromatographic method for the determination of triazine herbicides in natural water samples. Int J Environ Anal Chem 17:97–111

    Google Scholar 

  • Thurman EM, Meyer M, Pomes M, Prey CA, Schwab AP (1990) Enzyme-linked immunosorbent assay compared with gas chromatography/mass spectrometry for the determination of triazine herbicides in water. Anal Chem 62:2043–2048

    PubMed  Google Scholar 

  • Whittman C, Hock B (1989) Improved enzyme immunoassay for the analysis ofs-triazines in water samples. Food Agric Immunology 1:211–224

    Google Scholar 

  • Wilson MP, Savage EP, Adrian DD, Aaronson MJ, Keefe TJ, Hamar DH, Tessari JT (1987) Groundwater transport of the herbicide atrazine, Weld County, Colorado. Bull Environ Contam Toxicol 39:807–814

    PubMed  Google Scholar 

  • Wuest S, Doht U, Geirsch T, Whittmann C, Hock B (1990) Sensitives-triazine enzyme immunoassay for water samples in polystyrene tubes. GIT Fachz Lab 34:99–106

    Google Scholar 

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Authors and Affiliations

  1. Department of Food Science, University of Maine, 102 B Holmes Hall, 04469, Orono, Maine, USA

    Rodney J. Bushway & Lewis B. Perkins

  2. Department of Biochemistry, Institute of Chemical Technology, Prague, Czechoslovakia

    Ladislav Fukal

  3. ImmunoSystems Inc., 4 Washington Ave, 04074, Scarborough, Maine, USA

    Robert O. Harrison & Bruce S. Ferguson

Authors
  1. Rodney J. Bushway
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  2. Lewis B. Perkins
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  3. Ladislav Fukal
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  4. Robert O. Harrison
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  5. Bruce S. Ferguson
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Bushway, R.J., Perkins, L.B., Fukal, L. et al. Comparison of enzyme-linked immunosorbent assay and high-performance liquid chromatography for the analysis of atrazine in water from Czechoslovakia. Arch. Environ. Contam. Toxicol. 21, 365–370 (1991). https://doi.org/10.1007/BF01060357

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  • DOI: https://doi.org/10.1007/BF01060357

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