Abstract
The effect of Ca2+ by Ba2+ in artificial extracellular fluid on high-threshold inward calcium current at the neuronal somatic membrane was investigated in rat spinal ganglia using techniques of intracellular dialysis and voltage clamping. An increase in the Ba2+ conductance of high-threshold calcium channels was found, assessed by the increase in maximum current amplitude. The fall in the latter occurring during dialysis and connected with washout of intracellular contents was considerably retarded when Ca2+ was replaced by Ba2+, probably due to the removal of the blocking effect of intracellular Ca2+ on the high-threshold calcium channels. It was discovered that the connection between high-threshold calcium channels and cyclic nucleotide metabolism remained unimpaired when Ca2+ is replaced by Ba2+.
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A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 18, No. 3, pp. 313–318, May–June, 1986.
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Veselovskii, N.S., Fedulova, S.A. Effects of substituting barium for calcium ions during research into inward currents in mammalian neurons. Neurophysiology 18, 227–231 (1986). https://doi.org/10.1007/BF01052540
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DOI: https://doi.org/10.1007/BF01052540