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Isolierung, Reinigung and Charakterisierung einer endo-Pectinlyase (endo-PL)

Isolation, purification and characterization of an endo-pectinlyase (endo-PL)

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Summary

This paper describes an endo-pectinlyase isolated on a preparative scale from the commercial enzyme preparation Pectinex Ultra SP-L by using chromatofocusing. The most important biochemical and physico-chemical properties are presented. The purity of this endo-pectinlyase was proved by analytical isoelectric focusing and by typical biochemical reactions. The following constants were determined: pI 3.9, Km 1.91 mg/ml andV max0.88 min−1. The enzyme has a pH optimum of 6.0 with 71 % esterified apple pectin and of 8.5 with a highly (94%) esterified pectin. Temperature optima with these two substrates were 60° C and 65° C respectively. The enzyme showed a relatively high pH and temperature stability. Its activity was increased considerably in the presence of Na+ ions.

Zusammenfassung

In dieser Arbeit wurde mittels Chromatofocussierung eine endo-Pectinlyase (PL) impräparativen Maßstab aus dem technischen Enzympräparat Pectinex Ultra SP-L isoliert and durch die wichtigsten physikalisch-chemischen and biochemischen Eigenschaften charakterisiert. Ihre Reinheit wurde durch analytische isoelektrische Focussierung und typische biochemische Reaktionen nachgewiesen. Für die PL wurden folgende Konstanten bestimmt: IP 3,9;K m 1,91 mg/ml andV max 0,88/min. Die pH-Optima lagen bei Verwendung von Apfelpectin mit einem Veresterungsgrad (VE) von 71 % bzw. von hochver estertem Pectin (VE=94%) bei pH 6,0 bzw. bei pH 8,5, die Temperatur-Optima bei 60° C bzw. 65° C. Die PL wies eine relativ hohe Temperatur- und pH-Stabilität auf. Die PL-Aktivität wurde deutlich durch Na+-Ionen gesteigert.

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Authors and Affiliations

  1. Institut für Lebensmitteltechnologie, Universität Hohenheim, Garbenstrasse 25, D-7000, Stuttgart 70, Bundesrepublik Deutschland

    Rengang Zhou, Helmi Omran, Yushan Bai, Marco Tableros & Karlheinz Gierschner

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  1. Rengang Zhou
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  2. Helmi Omran
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  3. Yushan Bai
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  4. Marco Tableros
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  5. Karlheinz Gierschner
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Additional information

Fran Rengang Zhou, landwirtschaftliche Akademie Shijiazhuang Hebei (V.R. China), z. Zt. als Stipendiatin der chinesischen Regie rung an der Universitat Hohenheim

H. Omran, Suez Canal Universitat Ismailia (Agypten), z. Zt. als Stipendiat der Alexander von Humboldt-Stiftung an der Universitat Hohenheim

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Zhou, R., Omran, H., Bai, Y. et al. Isolierung, Reinigung and Charakterisierung einer endo-Pectinlyase (endo-PL). Z Lebensm Unters Forch 188, 551–556 (1989). https://doi.org/10.1007/BF01043049

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  • DOI: https://doi.org/10.1007/BF01043049

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