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The preparation of protein A-gold complexes with 3 nm and 15 nm gold particles and their use in labelling multiple antigens on ultra-thin sections

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Summary

The preparation of a protein A-gold complex (pAg3) using 3 nm gold particles and its application for labelling of intracellular antigens on thin sections is reported. The 3 nm gold particle is the smallest metal particle currently available for cytochemistry and permits a higher resolution of the pAg technique. Furthermore, it can be used in double labelling experiments in conjunction with a pAg complex prepared from 15 nm gold particles. For double labelling, the pAg3 complex must be used for staining of the first antigen since otherwise a non-specific co-labelling of the two pAg complexes results.

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  1. Institute of Histology and Embryology, University of Geneva Medical School, CH-1211, Geneva 4, Switzerland

    Jurgen Roth

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  1. Jurgen Roth
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Roth, J. The preparation of protein A-gold complexes with 3 nm and 15 nm gold particles and their use in labelling multiple antigens on ultra-thin sections. Histochem J 14, 791–801 (1982). https://doi.org/10.1007/BF01033628

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  • DOI: https://doi.org/10.1007/BF01033628

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