Summary
A Technicon AutoAnalyzer has been adapted to analyse lysine of fermentation broths from lysine-producer mutants, with a photometric method with ninhydrin. Linearity and accuracy have been tested, with errors lower than 5.5%. The great advantadge is its speed, analysing one sample every 5 minutes, automatically.
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References
Chinard, F.P. (1952)J. Biol. Chem. 199, 91–95.
Gale, E.F., (1967)in Methods of Biochemical Analysis (Glick, D., ed.), vol. IV, Interscience Publishers, New York.
Schaiberger, G.E., and Ferrari, A. (1960)N.Y. Acad. Sci. 87, 890–893.
Weber, R. (1972) Technical Rep. 1, Technicon Intern. Div., Ardsley, N.Y.
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Bordons, A. Automated determination of lysine by colorimetric method with ninhydrin. Biotechnol Lett 8, 411–414 (1986). https://doi.org/10.1007/BF01026742
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DOI: https://doi.org/10.1007/BF01026742