Summary
Thermophilic degradation of sugar beet pulp was studied in batch cultures at 55°C by different associations of bacteria, includingClostridium thermocellum,Methanobacterium sp. andMethanosarcina MP.C. thermocellum produced acetate, succinate, methanol, ethanol, H2 and CO2. The coculture ofC. thermocellum andMethanobacterium sp. produced trace amounts of ethanol and succinate; acetate concentration was about three times higher than in theC. thermocellum monoculture. The association of this coculture withMethanosarcina MP produced 5.5 mmol CH4/g dry weight sugar beet pulp.
Similar content being viewed by others
References
Arntz, H.J., Stoppok, E. and Buchholz, K. (1985) Biotechnol. Lett. 7, 113–118.
Cooney, C.R. and Wise, D.L. (1975) Biotechnol. Bioeng. 17, 1119–1135.
Garcia, J.L., Guyot, J.P., Ollivier, B., Trad, M. and Paycheng, C. (1982) Cah. ORSTOM, Sér. Biol. 45, 3–15.
Hanson, R.S. and Phillipps, J.A. (1981) In: Manual of Methods for General Microbiology. P. Gerhardt, R.G.E. Murray, R.N. Costilow, E.W. Nester, W.A. Wood, N.R. Krieg and G.B. Philipps, eds. pp. 328–364, ASM, Washington.
Khan, A.W. (1980) FEMS Microbiol. Lett. 9, 233–235.
Labat, M., Garcia, J.L., Meyer, F. and Deschamps, F. (1984) Biotechnol. Lett. 6, 379–384.
Laube, V.M. and Martin, S.M. (1983) Can. J. Microbiol. 29, 1475–1480.
Lescure, J.P. and Bourlet, P. (1984) Ind. Alim. Agric. 101, 601–607.
Mountfort, D.O., Asher, R.A. and Bauchop, J. (1982) Appl. Environ. Microbiol. 44, 128–134.
Ollivier, B., Lombardo, A. and Garcia, J.L. (1984) Ann. Microbiol. (Inst. Pasteur) 135 B, 187–198.
Ollivier, B., Mah, R.A., Garcia, J.L. and Robinson, R. (1985) Int. J. Syst. Bacteriol. 35, 127–130.
Schelef, G., Kimchie, S. and Grynberg, H. (1980) Biotechnol. Bioeng. Symp. 10, 341–351.
Schink, B. and Zeikus, J. G. (1980) Curr. Microbiol. 4, 387–389.
Stoppok, E. and Buchholz, K. (1985) Biotechnol. Lett. 7, 119–124.
Weimer, P.J. and Zeikus, J.G. (1977) Appl. Environ. Microbiol. 33, 289–297.
Wolin, M.J. (1974) Am. J. Clin. Nutr. 27, 1320–1328.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ollivier, B., Smiti, N. & Garcia, J.L. Thermophilic methanogenesis from sugar beet pulp by a defined mixed bacterial culture. Biotechnol Lett 7, 847–852 (1985). https://doi.org/10.1007/BF01025568
Issue Date:
DOI: https://doi.org/10.1007/BF01025568