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Utilization ofEscherichia coli tac promoter inBacillus subtilis

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Summary

The tac promoter originally constructed for the use inEscherichia coli was fused to an endoglucanase structural gene isolated fromBacillus subtilis and the expression of the chimeric gene inB. subtilis was observed. The tac promoter-controlled gene expressed well inB. subtilis and produced endoglucanase during the exponential growth phase.

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Authors and Affiliations

  1. Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Cheongryang, P. O. Box 150, 130-650, Seoul, Korea

    Baek Rak Lee & M. Y. Pack

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  1. Baek Rak Lee
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  2. M. Y. Pack
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Lee, B.R., Pack, M.Y. Utilization ofEscherichia coli tac promoter inBacillus subtilis . Biotechnol Lett 12, 925–930 (1990). https://doi.org/10.1007/BF01022592

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  • DOI: https://doi.org/10.1007/BF01022592

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