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Synthesis of eicosapentaenoyl phosphatidylcholines by polyethylene glycol-modified lipase in benzene

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Summary

Lipase fromCandida cylindracea was modified with activated polyethylene glycol, which was synthesized from α-carboxymethyl-ω-methoxypoly-(oxyethylene) and N-hydroxysuccinimide with dicyclohexylcarbodiimide. The modified lipase was soluble in organic solvents such as benzene and catalyzed the reaction of ester-exchange to synthesize eicosapentaenoyl phosphatidylcholines from dipalmitoyl phosphatidylcholine and eicosapentaenoic acid. Either one of palmitic acid at C-1 or C-2 position of the phosphatidylcholine was exchanged with eicosapentaenoic acid.

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Authors and Affiliations

  1. Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, Meguroku, Tokyo 152, Japan

    Takayuki Yoshimoto, Masahide Nakata, Shigehiko Yamaguchi, Tadashi Funada, Yuji Saito & Yuji Inada

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  1. Takayuki Yoshimoto
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  2. Masahide Nakata
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  3. Shigehiko Yamaguchi
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  4. Tadashi Funada
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  5. Yuji Saito
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  6. Yuji Inada
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Yoshimoto, T., Nakata, M., Yamaguchi, S. et al. Synthesis of eicosapentaenoyl phosphatidylcholines by polyethylene glycol-modified lipase in benzene. Biotechnol Lett 8, 771–776 (1986). https://doi.org/10.1007/BF01020820

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  • DOI: https://doi.org/10.1007/BF01020820

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