Summary
We have previously found that during exercise net muscle glycogen breakdown is impaired in adrenodemedullated rats, as compared with controls. The present study was carried out to elucidate whether, in rats with deficiencies of the sympatho-adrenal system, diminished exercise-induced glycogenolysis in skeletal muscle was accompanied by increased breakdown of triglyceride and/or protein. Thus, the effect of exhausting swimming and of running on concentrations of glycogen, protein, and triglyceride in skeletal muscle and liver were studied in rats with and without deficiencies of the sympatho-adrenal system. In control rats, both swimming and running decreased the concentration of glycogen in fast-twitch red and slow-twitch red muscle whereas concentrations of protein and triglyceride did not decrease. In the liver, swimming depleted glycogen stores but protein and triglyceride concentrations did not decrease. In exercising rats, muscle glycogen breakdown was impaired by adrenodemedullation and restored by infusion of epinephrine. However, impaired glycogen breakdown during exercise was not accompanied by a significant net breakdown of protein or triglyceride. Surgical sympathectomy of the muscles did not influence muscle substrate concentrations. The results indicate that when glycogenolysis in exercising muscle is impeded by adrenodemedullation no compensatory increase in breakdown of triglyceride and protein in muscle or liver takes place. Thus, indirect evidence suggests that, in exercising adrenodemedullated rats, fatty acids from adipose tissue were burnt instead of muscle glycogen.
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References
Baldwin KM, Reitman JS, Terjung RL, Winder WW, Holloszy JO (1973) Substrate depletion in different types of muscle and in liver during prolonged running. Am J Physiol 225: 1045–1050
Bancroft TA (1968) Topics in intermediate statistical methods. Iowa State Univ Press, Ames, p 101
Chernick SS (1969) Determination of glycerol in acyl glycerols. Methods Enzymol 14: 627–630
Decombaz J, Reinhardt P, Anatharaman K, von Glutz G, Poortmans JR (1979) Biochemical changes in a 100 km run: free amino acids, urea, and creatinine. Eur J Appl Physiol 41: 61–72
Dohm GL, Kasperek GJ, Tapscott EB, Beecher GR (1980) Effect of exercise on synthesis and degradation of muscle protein. Biochem J 188: 255–262
Dohm GL, Puente FR, Smith CP, Edge A (1978) Changes in tissue protein levels as a result of endurance exercise. Life Sci 23: 845–850
Dohm GL, Williams RT, Kasperek GJ, Van Rij AM (1982) Increased excretion of urea and N-methylhistidine by rats and humans after a bout of exercise. J Appl Physiol: Respirat Environ Exercise Physiol 52: 27–33
Essén B (1978) Studies on the regulation of metabolism in human skeletal muscle using intermittent exercise as an experimental model. Acta Physiol Scand [Suppl 454]
Jansson E, Kaijser L (1982) Effect of diet on the utilization of blood-borne and intramuscular substrates during exercise in man. Acta Physiol Scand 115: 19–30
Karlsson J, Diamant B, Saltin B (1971) Muscle metabolites during submaximal and maximal exercise in man. Scand J Clin Lab Invest 39: 179–183
Kasperek GJ, Dohm GL, Tapscott EB, Powell T (1980) Effect of exercise on liver protein loss and lysosomal enzyme levels in fed and fasted rats. Proc Soc Exp Biol Med 164: 430–434
Kissane JM, Robins E (1958) The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J Biol Chem 233: 184–188
Lemon PWR, Mullin JP (1980) Effect of initial muscle glycogen levels on protein catabolism during exercise. J Appl Physiol: Respirat Environ Exercise Physiol 48: 624–629
Lithell H, örlander J, Schele R, Sjödin B, Karlsson J (1979) Changes in lipoprotein-lipase activity and lipid stores in human skeletal muscle with prolonged, heavy exercise. Acta Physiol Scand 107: 257–261
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275
Masoro EJ, Rowell LB, McDonal RM (1966) Intracellular muscle lipids as energy sources during muscular exercise and fasting. Fed Proc 25: 1421–1424
Refsum HE, Gjessing LR, StrØmme SB (1979) Changes in plasma amino acid distribution and urine amino acids excretion during prolonged heavy exercise. Scand J Clin Lab Invest 39: 407–413
Rennie MJ, Edwards RHT, Krywawych S, Davies CMT, Halliday D, Waterlow JC, Millward DJ (1981) Effect of exercise on protein turnover in man. Clin Sci 61: 627–639
Richter EA, Galbo H, Sonne B, Holst JJ, Christensen NJ (1980) Adrenal medullary control of muscular and hepatic glycogenolysis and of pancreatic hormonal secretion in exercising rats. Acta Physiol Scand 108: 235–242
Richter EA, Galbo H, Christensen NJ (1981a) Control of exercise-induced muscular glycogenolysis by adrenal medullary hormones in rats. J Appl Physiol: Respirat Environ Exercise Physiol 50: 21–26
Richter EA, Sonne B, Christensen NJ, Galbo H (1981b) Role of epinephrine for muscular glycogenolysis and pancreatic hormonal secretion in running rats. Am J Physiol [Endocrinol Metab 3] 240: E526-E532
Snedecor GW, Cochran WG (1965) Statistical methods. Iowa State Univ Press, Ames, p 237, 251, 285
Stankiewicz-Choroszucha B, Gorski J (1978) Effect of beta-adrenergic blockade on intramuscular triglyceride mobilization during exercise. Experientia 34: 357–358
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Richter, E.A., Sonne, B., Mikines, K.J. et al. Muscle and liver glycogen, protein, and triglyceride in the rat. Europ. J. Appl. Physiol. 52, 346–350 (1984). https://doi.org/10.1007/BF01015225
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DOI: https://doi.org/10.1007/BF01015225