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Quantitative Analyse der Catecholamin-Biosynthese des Nebennierenmarks in vivo und Ruhesekretion neugebildeter Amine unter besonderer Berücksichtigung des Dopamins

Quantitative analysis of catecholamine biosynthesis in adrenal medulla in vivo and resting secretion of synthesized amines with special consideration of dopamine

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Summary

Cats and rabbits were injected intravenously with3H-tyrosine and the radioactivity of the catecholamines in adrenal glands and adrenal blood was measured up to 60 min after injection. Adrenal blood was collected in a cava pocket. In addition the specific activity of free3H-tyrosine in blood plasma was measured as a function of time.

Radioactive dopamine could be demonstrated in venous blood from adrenals of cat and rabbit by different methods (column chromatography with Dowex 50, paper chromatography, high voltage paper electrophoresis). On the other hand arterial blood flowing to the adrenals contained no3H-dopamine.

During the 30 min after the injection of3H-tyrosine cat adrenals secreted about 14% of the synthesized radioactive catecholamines as3H-dopamine and 8% as3H-noradrenaline. In contrast to this the adrenals of rabbits secreted not more than 3% as3H-dopamine and about 20% as3H-noradrenaline.

The catecholamine biosynthesis rate of the adrenals and their dopamine resting secretion was estimated from the specific activity of3H-tyrosine in blood plasma and the radioactivity of catecholamines in adrenal glands and adrenal blood. In cats two adrenals synthesized 0.75 mμMol/min catecholamine per kg body weight and in rabbits 0.074 mμMol/min/kg body wt. respectively. The dopamine resting secretion in cats was about 0.08 mμMol/min and in rabbits about 0.002 mμMol/min.

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Authors and Affiliations

  1. Institut für Medizinische Strahlenkunde der Universität Würzburg, Deutschland

    Klaus Hempel & Heinrich F. K. Männl

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  1. Klaus Hempel
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  2. Heinrich F. K. Männl
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Über einen Teil der Ergebnisse wurde in Form einer Kurzmitteilung (Hempel u.Männl, 1967 a) und auf der 32. Tagung der Deutschen Pharmakologischen Gesellschaft vorgetragen (Hempel u.Männl, 1969).

Herrn Prof. Dr. W.Maurer danken wir für die stets großzügige Förderung der Arbeit. FräuleinRegina Kreutz, FrauUrsula Stehr, FräuleinGisela Döllscher und FräuleinGisela Bingmann sind wir für interessierte Mitarbeit zu Dank verpflichtet. Noradrenalin und Adrenalin wurden von den Farbwerken Hoechst AG und ein Teil des Tiermaterials von der Fa. Dr. Madaus Co., Köln-Merheim, freundlicherweise zur Verfügung gestellt. Der Deutschen Forschungsgemeinschaft danken wir für die Gewährung von Sach- und Personalmitteln.

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Hempel, K., Männl, H.F.K. Quantitative Analyse der Catecholamin-Biosynthese des Nebennierenmarks in vivo und Ruhesekretion neugebildeter Amine unter besonderer Berücksichtigung des Dopamins. Naunyn-Schmiedebergs Arch. Pharmak. 264, 363–388 (1969). https://doi.org/10.1007/BF01002377

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  • DOI: https://doi.org/10.1007/BF01002377

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