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Adrenergic receptors coupled to adenylate cyclase in human cerebromicrovascular endothelium

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Abstract

Cultured endothelium derived from three microvascular fractions of human brain was used to characterize adrenergic receptors coupled to adenylate cyclase activity. Catecholamines (norepinephrine, epinephrine) and their analogs (isoproterenol, phenylephrine, 6-fluoronorepinephrine) dose-dependently stimulated endothelial production of cAMP. Antagonists for ß1 and ß2receptors (propranolol, atenolol, and butoxamine) and for α1-receptors (prazosin) dose-dependently blocked cAMP formation induced by the tested adrenergic agonists. Clonidine, an ga2>α1-agonist, also inhibited isoproterenol-stimulated production of cAMP while yohimbine (α2>α1 antagonist) augmented the norepinephrine or epinephrine-induced accumulation of cAMP. Cholera toxin-induced ADP ribosylation of the stimulatory guanine nucleotide binding protein (Gs) abolished the stimulatory effect of norepinephrine, epinephrine, phenylephrine or 6-fluoronorepinephrine on cAMP formation. ADP ribosylation of the inhibitory guanine nucleotide binding protein (Gi) by pertussis toxin had no effect on either phenylephrine-or 6-fluoronorepinephrine-induced production of cAMP while it increased the norepinephrine and epinephrine-induced accumulation of cAMP. These findings represent the first documentation of ß1-, ß2-, α1 and α 2-adrenergic receptors linked to adenylate cyclase in endothelium derived from human brain microvasculature. These data also indicate that activation of endothelial α1 -adrenergic receptors is mediated by a signal transduction mechanism associated with Gs protein. The results strongly support the presence of various receptor-controlled adrenergic regulatory mechanisms on human cerebromicrovascular endothelium.

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Authors and Affiliations

  1. Stroke Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD

    Fatima Bacic, Richard M. McCarron & Maria Spatz

  2. The Johns Hopkins Hospital, Baltimore, MD

    Sumio Uematsu

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  1. Fatima Bacic
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  2. Richard M. McCarron
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  3. Sumio Uematsu
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  4. Maria Spatz
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Bacic, F., McCarron, R.M., Uematsu, S. et al. Adrenergic receptors coupled to adenylate cyclase in human cerebromicrovascular endothelium. Metabolic Brain Disease 7, 125–137 (1992). https://doi.org/10.1007/BF01000158

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  • DOI: https://doi.org/10.1007/BF01000158

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